| Title | Description | Published | Added |
| Ep 10 Part 1: Kristine Bunch's Nightmare | In part 1 of this episode, you'll hear about one woman's nightmare come true. In 1995, 21-year-old Kristine Bunch was abruptly awoken in the early morning hours to discover that her house was on fire. After escaping the flames, she watched in horror as firefighters were unable to save her 3-year-old son who was still trapped inside. Within hours of being released from the hospital, she discovered that detectives had a theory about the fire - someone had started the fire on purpose, and their main suspect was Kristine. A special thank you to Kristine Bunch for helping me tell her story.Please consider supporting Court Junkie with as little as $3 a month via Patreon.com/CourtJunkie. Help support Court Junkie with $6 a month and get access to bonus monthly episodes.Follow me on Twitter @CourtJunkiePod or Instagram at CourtJunkie. Music featured in this episode: Like Music (cdk Mix) by cdk (c) copyright 2015 Licensed under a Creative Commons Attribution Share-Alike (3.0) license. htt; | 2016 | 2023-07-08T12:43:39Z |
| Indian rubber man | | 1939 | 2025-09-09T22:39:54Z |
| In re: Dealer Management Systems Antitrust Litig. v. CDK Global, LLC | This item represents a case in PACER, the U.S. Government's website for federal case data. This information is uploaded quarterly. To see our most recent version please use the source url parameter, linked below. To see the canonical source for this data, please consult PACER directly. | | 2020-03-22T17:58:37Z |
| The Old Architecture of Quebec | | 1947 | 2025-05-22T17:20:02Z |
| treatment of the patient past fifty | | 1941 | 2025-09-04T01:47:40Z |
| Everyday Things In American Life | | 1941 | 2025-09-09T13:08:10Z |
| Indian Oasis | | 1939 | 2025-08-24T05:44:54Z |
| Coombs v. CDK Global, LLC | This item represents a case in PACER, the U.S. Government's website for federal case data. If you wish to see the entire case, please consult PACER directly. | | 2024-09-30T09:06:18Z |
| Proto v. CDK Global, LLC | This item represents a case in PACER, the U.S. Government's website for federal case data. If you wish to see the entire case, please consult PACER directly. | | 2024-07-25T09:17:26Z |
| github.com-sindresorhus-awesome_-_2022-08-11_12-20-41 | 😎 Awesome lists about all kinds of interesting topics Check out: One Thing — Put a single task or goal in your menu bar My open source work is supported by the community Special thanks to: All your environment variables, in one place Stop struggling with scattered API keys, hacking together home-brewed tools, and avoiding access controls. Keep your team and servers in sync with Doppler. Your app, enterprise-ready. Start selling to enterprise customers with just a few lines of code. Add Single Sign-On (and more) in minutes instead of months. Strapi is the leading open-source headless CMS. It’s 100% JavaScript, fully customizable, and developer-first. Founded in 2018, OSS Capital is the first and only venture capital platform focused exclusively on supporting early-stage COSS (commercial open source) startup founders. Instant, shareable cloud PostgreSQL database Import any dataset in seconds, share with anyone with a click, try without signing up Dev environments built for the clo; | 2022 | 2022-08-18T11:22:09Z |
| Matos v. CDK Global, LLC | This item represents a case in PACER, the U.S. Government's website for federal case data. This information is uploaded quarterly. To see our most recent version please use the source url parameter, linked below. To see the canonical source for this data, please consult PACER directly. | | 2018-10-05T16:04:41Z |
| High p27 protein levels in chronic lymphocytic leukemia are associated to low Myc and Skp2 expression, confer resistance to apoptosis and antagonize Myc effects on cell cycle. | This article is from Oncotarget , volume 5 . Abstract Myc (c-Myc) counteracts p27 effects, and low p27 usually correlates with high Myc expression in human cancer. However there is no information on the co-expression of both genes in chronic lymphocytic leukemia (CLL). We found a lack of correlation between RNA and protein levels of p27 and Myc in CLL cells, so we determined the protein levels by immunoblot in 107 cases of CLL. We observed a high p27 protein expression in CLL compared to normal B cells. Ectopic p27 expression in a CLL-derived cell line resulted in cell death resistance. Surprisingly, Myc expression was very low or undetectable in most CLL cases analyzed, with a clear correlation between high p27 and low Myc protein levels. This was associated with low Skp2 expression, which is consistent with the Skp2 role in p27 degradation and with SKP2 being a Myc target gene. High Myc expression did not correlate with leukemia progression, despite that cell cycle-related Myc target; | 2014 | 2014-10-06T05:32:23Z |
| CIA Reading Room cia-rdp91-00403r000200380008-6: FR:IZSO L TO:BAGER G | Traffic Organization and Preparation of Tim Tableofor Pusenger Shippliig on the Balaton.MMGARIANI perk Kozlekedese KozIony) 24 may 1970.,PP 384-387.DTA LN 1328-70Ju1Y 71The Plans and Tasks of Mobart for the Year 1970.RMARIM., per. KozIekedesi Kbzlony, No 16,19 April 1970, p 285.DTA rX 114g-7oJ'AlY 71Trends In Transportmbion and Postal Output Devicing1969.MMGMIM~ perk Kozelekedsi lCozIony, No 153April 12, 1970, p~ M7-2',11.DIA LN 1148-70May 71The Twerity-Five Years of Hungarian Shipping.UMMIM, per, I-a-ziekedesi KoziMp .4 April 1970sPp 248-250-DiA Lw 1147-70State PAIUVWU# IM Task* MftbWobyWay &*Wlo 9 pp.HUWARW Paogo 90 IYAOr 0ins 50417RF,NungaryF400&F ?0 407j,346V^aq;mBow&r 70 4020445NW& 7111 lans for I~ailroad Containerization wotexi.by Laszlo Izao, k~yorgy ionyrd, 9 pp,10WARIAW, por, Xozlq)cedAk 1~q pnv, lWapust,30 I'~ov 1969. pp W~Z~d- L4 _-i ei~s 49688U~ImngaryBconi-eb 70 40o,6o,6-laticnal aid i iiaij)our L,; 40I )ILL.14lUji"'It"L.'j, ~ILEi2!L~ 17 ~.;,),vo, 1'J69Ilil.,\ 1~~ U48-69(D; | 1983 | 2026-03-31T13:43:14Z |
| Sedimentary Petrography | | 1929 | 2025-09-11T12:09:08Z |
| Het Utrechts Archief 168792 | Het Utrechts Archief catalog number: 168792 Description (Dutch): Afbeelding van een gezelschap op het 4e perron van het N.S.-station Rotterdam C.S. te Rotterdam, bij het electrische treinstel nr. 461 (mat. 1936) van de N.S.N.B. Het betreft hier een gezelschap uit een extra trein voor de Handelsmaatschappij R.S. Stokvis en Zonen. Het treinstel 461 was een electrisch drierijtuigen-treinstel dat na de oorlog is samengesteld uit bakken van diverse treinstellen (Bk 607, C 613 en CDk 610). | 1961 | 2021-02-05T03:16:03Z |
| github.com-duo-labs-cloudtrail-partitioner_-_2019-11-08_14-09-19 | None This project sets up partitioned Athena tables for your CloudTrail logs and updates the partitions nightly. As new AWS accounts begin sending you logs or new AWS regions come online, your paritions will always be up-to-date. It is based on work by Alex Smolen in his post Partitioning CloudTrail Logs in Athena . You can immediately deploy the CDK app, but I recommend first running this manaully to ensure everything is configured, and also because running it manually will (by default) create 90 days of partitions, whereas the nightly CDK will not run until 0600 UTC, and will only create partitions for the current day and tomorrow. Tables are created for each account as cloudtrail_000000000000 and also a view is created that unions all these tables. Setup Edit config/config.yaml to specify the S3 bucket containing your CloudTrail logs, the SNS to send alarms to (you must create one if you don't already have one) and any other configuraiton info. Set up the initial tables and partitio; | 2019 | 2019-12-29T22:19:50Z |
| github.com-awslabs-aws-cdk_-_2019-07-04_08-54-12 | The AWS Cloud Development Kit is a framework for defining cloud infrastructure in code AWS Cloud Development Kit (AWS CDK) The AWS Cloud Development Kit (AWS CDK) is an open-source software developmentframework to define cloud infrastructure in code and provision it through AWS CloudFormation.The CDK integrates fully with AWS services and offers a higher level object-orientedabstraction to define AWS resources imperatively. Using the CDK’s library ofinfrastructure [constructs], you can easily encapsulate AWS best practices in yourinfrastructure definition and share it without worrying about boilerplate logic. TheCDK improves the end-to-end development experience because you get to use the powerof modern programming languages to define your AWS infrastructure in a predictableand efficient manner. The following languages are currently supported: JavaScript, TypeScript Python Java .NET The CDK is currently in developer preview and we look forward to community feedback and collaboration!; | 2019 | 2019-07-04T09:05:48Z |
| github.com-debezium-debezium_-_2021-12-09_23-05-13 | Change data capture for a variety of databases. Please log issues at https://issues.redhat.com/browse/DBZ. Copyright Debezium Authors.Licensed under the Apache License, Version 2.0 .The Antlr grammars within the debezium-ddl-parser module are licensed under the MIT License . English | Chinese | Japanese Debezium Debezium is an open source project that provides a low latency data streaming platform for change data capture (CDC). You setup and configure Debezium to monitor your databases, and then your applications consume events for each row-level change made to the database. Only committed changes are visible, so your application doesn't have to worry about transactions or changes that are rolled back. Debezium provides a single model of all change events, so your application does not have to worry about the intricacies of each kind of database management system. Additionally, since Debezium records the history of data changes in durable, replicated logs, your application can be stoppe; | 2021 | 2021-12-09T23:51:39Z |
| Dual Control of Yen1 Nuclease Activity and Cellular Localization by Cdk and Cdc14 Prevents Genome Instability. | This article is from Molecular Cell , volume 54 . Abstract The careful orchestration of cellular events such as DNA replication, repair, and segregation is essential for equal distribution of the duplicated genome into two daughter cells. To ensure that persistent recombination intermediates are resolved prior to cell division, the Yen1 Holliday junction resolvase is activated at anaphase. Here, we show that the master cell-cycle regulators, cyclin-dependent kinase (Cdk) and Cdc14 phosphatase, control the actions of Yen1. During S phase, Cdk-mediated phosphorylation of Yen1 promotes its nuclear exclusion and inhibits catalytic activity by reducing the efficiency of DNA binding. Later in the cell cycle, at anaphase, Cdc14 drives Yen1 dephosphorylation, leading to its nuclear relocalization and enzymatic activation. Using a constitutively activated form of Yen1, we show that uncontrolled Yen1 activity is detrimental to the cell: spatial and temporal restriction of Yen1 protects against g; | 2014 | 2014-10-23T07:40:09Z |
| yahoo-groups-2018-07-22T15-57-51Z-09afd9 | Yahoo! Groups API grab. Contains messages of the following groups: 1cemerlang_cikguyatie 1st0910rc301_mpe_rdpou 212d03_ics_1stsem2009 225x01_ess_1stsem2009 26auxiliary 4s4_ihc_diplomacy a1amsterdam acupuntura01_incisa-sp aiesecateneoapplicants0910 alanatura alumni787 alumni_kursus_pplh_ipb amocabelo angkan_ni_nita_at_totoy atmku basicon_hr101p bassettrd beauty-of-nature bioclass_sjcbalanga bolitas_5 bshrm3b_csu bsn_tri-b12 btrsport cdd_comp_comm_109 cdk_database_system cdplayers cg_services citsrtece cmsfac cnpbr collinsresearcher constanciaesperanca constructiontoysuperstation copro1_bs102a cs30dbloggroup cso_09 cuetcse_10yearsanniv curvydiamonddivaschicago cwts1_complab cwts1i_gkhousebuild09 cwts-sw1 denvershuls desisofgreaterdayton dlspbeccounselors dlsplearningleaderstec2 dragonsoffirevalley ds2013 dungeonsofeberron eeplangkawi09 elite3rcbc emsa_rostock euusmilliondollarlicensing ff_09-10 fhunpad04 fineartsmhc1 firstsemsy0910mth2ndclass forte_mens_chorus forum_editores gameigamel g; | | 2018-07-22T15:58:00Z |
| yahoo-groups-2018-09-04T07-32-48Z-3ec9b9 | Yahoo! Groups API grab. Contains messages of the following groups: 9csmpdobozmanado abu3loush adm_etec_malu adrian-umina ailiv_tennis alumnisma7bdg80 americanfieldhockey anne_ssa anotherm3group arduinohome arianaprfghanipoursummer2010 arrkoola_uq asma_feab asolidstepforward asorii_2010 asra_group bedogjakarta bellocienciacriminal bge07_ku bianchigil bp_pta_board_10-11 bte2006-2010 caribbeanpirates390 ccc-philippines cdk_dsp ceni_meeting centralboard_usteduc clubfetichistademexico colmbankfact coup_depouce cpaf-gsaofficers2010-2011 d1015westernchampion dailyhotactress ddfep10kce dil_se_delhi ea2010_pubpromo eccwomenclergy education_batch25a edu_infantil_sme empregos_vagas_rj enfermagem_unoesc02 escuelatv02 espeflorania ethnopharma_uoda falconcoachken fciprog fil11-bbb forum_agenda21_cidadeademar fossbbsr gaqhxm gctalumni-bangalorechapter genocidio_e_politica gmcs_july2010 graycouncilofarnor gudgurlzzz iba_bicolanos_in_la ibanezblack igrab-2012mbc-bid iiucete imt_tacvps indonesia_sde_com; | | 2018-09-04T07:32:59Z |
| Anti-Cancer Effect of IN-2001 in T47D Human Breast Cancer. | This article is from Biomolecules & Therapeutics , volume 20 . Abstract Histone deacetylases (HDACs) are enzymes involved in the remodelling of chromatin, and have a key role in the epigenetic regulation of gene expression. Histone deacetylase (HDAC) inhibitors are emerging as an exciting new class of potential anti-cancer agents. In recent years, a number of structurally diverse HDAC inhibitors have been identified and these HDAC inhibitors induce growth arrest, differentiation and/or apoptosis of cancer cells in vitro and in vivo. However, the underlying molecular mechanisms remain unclear. This study aimed at investigating the anti-tumor activity of various HDAC inhibitors, IN-2001, using T47D human breast cancer cells. Moreover, the possible mechanism by which HDAC inhibitors exhibit anti-tumor activity was also explored. In estrogen receptor positive T47D cells, IN-2001, HDAC inhibitor showed anti-proliferative effects in dose-and time-dependent manner. In T47D human breast cancer; | 2011 | 2014-10-24T20:14:53Z |
| Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication. | This article is from The Journal of Cell Biology , volume 193 . Abstract Phosphorylation of human Treslin by Cdk is essential for its association with TopBP1 and for the initiation of DNA replication in S phase. | 2011 | 2014-10-25T19:09:59Z |
| Black Branches: A Book of Poems and Plays | Book digitized by Google from the library of the University of California and uploaded to the Internet Archive by user tpb. | 1920 | 2008-09-02T09:35:55Z |
| DTIC ADA407447: Role of Cyclin D1 and cdk Inhibitors in Breast Cancer Pathogenesis | Cyclin Dl is frequently overexpressed in breast cancer tumors. Thus, an understanding of cyclin Dl regulation in normal and malignant cells may prove beneficial in the search for therapeutic targets. In fibroblastic cell lines, cyclin Dl expression is regulated by Rho/Rho kinase: i) when Rho/Rho kinase is active, ERK activity is sustained and consequently cyclin Dl is induced in mid-G1 phase, and ii) when Rho/Rho kinase is inactive, cyclin Dl is induce in early G1 phase through a rac-mediated pathway (implicating PI-3 kinase signaling) . In this report, I examined the consequences and regulation of rac-mediated cyclin Dl expression in terms of Rho kinase-dependent stress fiber formation. Selective inhibition of Rho kinase allowed for rac-mediated cyclin Dl expression, the downregulation of p21ci pl% %% and p27 kiPl and S phase entry, indicating that cell proliferation can occur under conditions where stress fiber formation is. compromised. The suppress ion of the rac pathway to cyclin ; | 2002 | 2018-05-09T21:16:49Z |
| DTIC ADA398172: Role of c-Myc in Estrogen Stimulated Cell Proliferation in Normal and Malignant Breast Epithelial Cells | Estrogenic steroids have diverse physiological functions and are critically involved in the pathogenesis of breast cancer. The transcriptional regulator c-Myc has been implicated in estrogen-induced mitogenesis and we are therefore investigating the contribution of c-Myc to various downstream molecular and cellular events after estrogen stimulation. We have now developed a series of cell lines expressing c-Myc and mutants lacking one or both of the conserved 'Myc boxes' required for transcriptional regulation activity, under the control of an inducible promoter. Ongoing experiments are examining whether these mutants can mimic the effects of estrogen in activating cyclin E-Cdk2 and inducing cell cycle progression. We have recently shown that decreased expression of the CDK inhibitor p21, a c-Myc target, is critical for estrogen activation of cyclin E-Cdk2 and consequently the effect of the c-Myc mutants on p21 expression is of particular interest. We have also shown that decreased c-My; | 2001 | 2018-05-05T20:39:40Z |
| The sacramewnts in the new testament | | 1903 | 2025-05-22T17:11:10Z |
| Priority 1 Automotive Group, Inc. v. CDK Global, LLC | This item represents a case in PACER, the U.S. Government's website for federal case data. If you wish to see the entire case, please consult PACER directly. | | 2021-11-18T14:05:17Z |
| Hopkins v. CDK Global, Inc. | This item represents a case in PACER, the U.S. Government's website for federal case data. If you wish to see the entire case, please consult PACER directly. | | 2022-05-06T09:18:12Z |
| Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1?MYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase. | This article is from Biochemical Journal , volume 461 . Abstract NUAK1 (NUAK family SnF1-like kinase-1) and NUAK2 protein kinases are activated by the LKB1 tumour suppressor and have been implicated in regulating multiple processes such as cell survival, senescence, adhesion and polarity. In the present paper we present evidence that expression of NUAK1 is controlled by CDK (cyclin-dependent kinase), PLK (Polo kinase) and the SCFβTrCP (Skp, Cullin and F-boxβTrCP) E3 ubiquitin ligase complex. Our data indicate that CDK phosphorylates NUAK1 at Ser445, triggering binding to PLK, which subsequently phosphorylates NUAK1 at two conserved non-catalytic serine residues (Ser476 and Ser480). This induces binding of NUAK1 to βTrCP, the substrate-recognition subunit of the SCFβTrCP E3 ligase, resulting in NUAK1 becoming ubiquitylated and degraded. We also show that NUAK1 and PLK1 are reciprocally controlled in the cell cycle. In G2–M-phase, when PLK1 is most active, NUAK1 levels are low and ; | 2014 | 2014-10-10T19:09:13Z |
| Nov 04 2025 Town meeting | | 2025 | 2025-11-06T13:34:51Z |
| DTIC ADA366953: General Methods for Identifying Gl-phase Substrates of Cdk Protein Kinases. | We are developing methods to identify Gi-phase substrates of cyclin-Cdk: complexes. In earlier years of this project, we optimized two-dimensional gels for visualization of phosphorylation of Cdc28 substrates, and we developed monoclonal antibodies with reactivity to phospho-Ser-Pro or phospho-Thr- Pro. We identified one important in vivo substrate, Sic 1. However, it appears that these methods by themselves are insufficiently powerful to identify many substrates, since most substrates seem to be very rare. We have therefore supplemented these methods with three other approaches: (1) purification of affinity tagged cyclins, to look for co-purifying proteins; (2) complete genome two-hybrid screens with Cdc28 and with Gi cyclins, to look for interacting proteins; and (3) genome-wide analysis of cell cycle regulated transcription, on the basis that some important substrates might be regulated at the transcriptional level as well as at the activity level. All three approaches are going ver; | 1998 | 2018-04-24T23:15:05Z |
| DTIC AD1034353: CDK5 A Novel Role in Prostate Cancer Immunotherapy | Our project will establish the role of CDK5 in promoting the immunosuppressive microenvironment in prostate cancer, and identify optimal strategies for incorporation of CDK5 inhibition to augment the efficacy of immunotherapy for prostate cancer. We will confirm our observation of the involvement of a T cell antitumor response in impaired growth of prostate cancer in immunocompetent murine models of prostate cancer, and characterize the changes induced in immune cells in the tumors. Preclinical translational studies, employing a CDK5 inhibitor in combination with immunotherapies, including immune checkpoint blockers, a prostate cancer vaccine, and other agents will be conducted and optimized in vivo in an immunocompetent prostate cancer model. If successful, these therapeutic strategies can be rapidly advanced to clinical evaluation. In this reporting period, our most significant finding was that depletion of CD4+ and CD8+ T cells resulted in more rapid tumor growth, and significant sh; | 2016 | 2020-03-23T23:34:41Z |
| CIA Reading Room cia-rdp83m00914r002300100097-4: PUBLICATION OF UNCLASSIFIED ANALYSIS | Approved For Release 2007/03/09: CIA-RDP83MOO914R002300100097-4 .xcacutlve Rayic4ig FEB 17 4 05 P4 ?8L MEMORANDUM FOR: Director of Central Intelligence OEXA 82-0455/1 17 FEB 1982 Director, ice o External Affairs SUBJECT: Publication of Unclassified Analysis 1. Action. Requested: Sign the attached letter to Gustav F. Papanek, Boston University, President of the Association for .Comparative Economic Studies. 2. Background: Pananek wrote you on 5 February 1982 to ask that you resume release of unclassified. CIA analysis to the public. (Tab A) The attached proposed response is consistent with your 20 January 1982 letter to Messrs. Becker and Horelick at the.Rand Corporation, and a more recent letter Bob Gates sent to Dr. Grossman of the University.of California on the same subject. (Tab B) It has been adjusted to avoid the look of a form letter, however, since it is likely that these people exchange letters with each other. 3. Recommendation: Sign the attached letter. U -:, Ui1- Director o; | 1982 | 2025-02-22T15:23:14Z |
| github.com-tiantianGPU-reg-factory_-_2026-06-04_06-12-01 | None # 🏭 reg-factory### Outlook · ChatGPT · Grok · Claude 全自动注册机**自动批量注册 Outlook 邮箱 → 用它在 ChatGPT / Grok / Claude 上注册账号 → 导出可直登 cookie** reg-factory 是一套全自动注册流水线：先自注册 Outlook 邮箱，再用同一邮箱在 ChatGPT / Grok / Claude 上批量注册账号，并导出可直登的 cookie。底层用 比特浏览器(BitBrowser) 做指纹隔离、 Clash Verge 做节点切换绕区域封锁与 Cloudflare 风控、接码/打码平台过手机号与验证码。 ⚠️ 仅供学习与授权测试使用。所有密钥通过环境变量提供，仓库内不含任何明文凭据。 1. 前置条件 ① 比特浏览器 BitBrowser 安装并 启动 比特浏览器客户端，确保本地 API 在线（默认 http://127.0.0.1:54345 ）。 客户端要保持运行——脚本通过该 API 创建/打开/关闭浏览器窗口。 ② Clash Verge（开启 API 权限） 安装 Clash Verge 并导入你的机场; | 2026 | 2026-06-04T22:05:03Z |
| The Early theory of equations : on their nature and constitution : translations of three treatises | 218 p. ; 23 cm // On the recognition of equations / by François Viète -- A new discovery in algebra / by Albert Girard -- On the nature and constitution of equations / by Florimond de Beaune | 1986 | 2023-12-22T11:17:45Z |
| DTIC ADA384004: Regulation of Ubiquitin Mediated Proteolysis of G1 Cyclins and the CDK Inhibitor p27 by the Cullin Gene Family in Normal and Tumorigenic Human Breast Cells | Sequential activation and inactivation of cyclin dependent kinases (CDK) regulate eukaryotic cell cycle transitions. The periodicity of CDK activity provides a molecular basis for unidirectional cell cycle progression and is partly controlled by ubiquitin-mediated proteolysis of both cyclins and CDK inhibitors. Deregulated expression of G1 CDK inhibitors and G1 cyclins have been directly linked to breast cancer development. The mechanisms regulating the ubiquitination of these two protein families constitute essential components of G1 cell cycle control in mammalian cells, but they are poorly understood at present. Polyubiquitination of proteins is known to be catalyzed by a cascade of enzymes: El (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligating). Two issues critical to our understanding of the regulation of protein turnover are how E3 ligases target proteins for ubiquitination and how they themselves are regulated. We have found that cullins partner with ; | 1999 | 2018-04-30T00:33:35Z |
| DTIC ADA386852: Role of cdc25 Phosphatases in Cellular Immortalization | The goal of this proposal is to study the potential role of tyrosine phosphorylation-dependent checkpoint and especially the cdc25 phosphatase family of cyclin dependent kinase (CDK) activators in cellular immortalization. We have shown that cdc25 phosphatases did in fact extend the normal mammary epithelial cells (HMEC) life span, but do not immortalize them. The effect was most prominent with cdc25A and cdc25B phosphatases. Cdc25C phosphatase had less effect on HMEC life span. Experiments with catalytically inactive cdc25A mutant show that phosphatase activity of cdc25A is essential for the life span extension. Our experiments show that telomerase is not activated by cdc25A expression in normal human cells, suggesting that cdc25 most likely affect Ml, but not M2 checkpoints. Retroviral cDNA libraries (sense and anti-sense) were introduced into HMEC together with cdc25 to assay for the potential enhancement of cdc25 ability to expand the cellular life span. Work is underway to identif; | 2000 | 2018-04-30T22:25:18Z |
| DTIC ADB281573: Role of Cyclin D1 and cdk Inhibitors in Breast Cancer Pathogenesis | PI3K signaling is involved in many aspects of cell cycle progression and is often deregulated in malignant cells. Akt, a downstream kinase effector of PI3K, stabilizes cyclin Dl protein and phosphorylates the cdk inhibitor p21 preventing its anti-proliferative effects in breast cancer cells. In this report we attempt to identify PI3K- dependent molecules necessary to rescue cyclin A expression. We examined if loss of p27 together with forced expression of cyclin Dl was sufficient to rescue cyclin A expression in p27-null MEFs and demonstrated that S phase entry could not be restored by forced cyclin Dl expression. As it has been reported that the cyclin A promoter contains a cyclic AMP responsive element and that Akt phosphorylates Ser-133 of CREB in response to mitogens, we examined the phosphorylation state of CREB in the presence or absence of LY. No significant differences in CREB activation were observed suggesting additional transcription factors are required for PI3K effects on ; | 2001 | 2018-03-19T02:29:50Z |
| The Harvard Classics : Vol. 2: Plato, Epictetus, Marcus, Aurelius | | 1909 | 2023-11-06T15:11:16Z |
| Het Utrechts Archief 163904 | Het Utrechts Archief catalog number: 163904 Description (Dutch): Afbeelding va een aantal monteurs tijdens werkzaamheden aan het draaistel van de CDk-bak van het electrische treinstel nr. 657 (mat. 1946) van de N.S. in de revisieloods van de werkplaats Zaanstraat te Amsterdam. | 1950 | 2021-02-24T23:03:58Z |
| DTIC ADA302241: The Role of Basic Fibroblast Growth Factor in Human Breast Cancer. | We found that basic fibroblast growth factor (bFGF), a mitogen, inhibits human breast cancer cell lines. In MCF-7 cells, bFGF binds high-affinity tyrosine kinase FGF receptors (FGFR), activates MAP kinase, induces higher levels of G(1) cyclins D1 and E and cyclin dependent kinase (cdk) 4, but causes G(1) cycle arrest through induction of cdk inhibitor p2l(WAFl/CIPl) FGF increases association of p21 with complexes of cyclins D1 and E, inactivates cdk2 and dephosphorylates retinoblastoma protein (Rb). FGF inhibits other mammary cell lines, but only if they do not contain cytoplasmic (18 kD) and nuclear localizing (22 and 24kD) bFGF. MCF-7 cells engineered to express all three bFOF moieties (MCF-7/NCF) secreted all forms and could not bind exogenous 18 kD bFGOF. They were arrested in G(1), had constitutive heterodimerization between FGFR1 and FGFR4, did not phosphorylate MAP kinase, had the same levels of cyclins D1 and E, cdk4 and p21(WAFl/CIPl) as controls, did not inactivate cdk2 nor d; | 1995 | 2018-03-25T17:34:48Z |
| DTIC ADB265310: Role of Cyclin D1 and cdk Inhibitors in Breast Cancer Pathogenesis | Extensive molecular, biochemical, cell biological, and cytogenetic studies of breast cancer cell lines and breast cancer biopsies have indicated that overexpression of cyclin Dl plays an important role in breast tumor oncogenesis. Particularly, D-type cyclins are fundamental to cell cycle regulation of the tumor suppressor protein, Rb. However, cyclin Dl has been reported being overexpressed to the same degree in both the early and late stages of breast cancer. Therefore, cyclin Dl overexpression alone may not be a good indicator of breast cancer. Preliminary data from our laboratory suggest that the cdk inhibitor p21(cipl) can be upregulated bycyclin Dl overexpression in NIH3T3. Also, we are examining the role of PI3K pathway in cell cycle progression given its relevance in cyclin Dl expression and its correlation with the tumor supressor gene, PTEN. We show in MEFs that LY294OO2 (LY), a PI3K inhibitor, is able to reduce cyclin Dl expression level and to block entry in S phase with li; | 2000 | 2018-03-11T00:30:27Z |
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| github.com-DovAmir-awesome-design-patterns_-_2021-07-10_17-58-07 | A curated list of software and architecture related design patterns. Awesome Software and Architectural Design Patterns A curated list of software and architecture related design patterns. Software design pattern - A general, reusable solution to a commonly occurring problem within a given context in software design. It is a description or template for how to solve a problem that can be used in many different situations. Contents Programming language design patterns General Architecture Cloud Architecture Serverless Architecture Micro services & Distributed Systems Internet of things Big Data Databases and storage Docker and DevOps Mobile Front End Development Security Books Programming Language Design Patterns AngularJS design patterns C# design-patterns design-patterns-for-human - ultra simplified explanation to design patterns. Design Patterns Library C++ design-patterns Closure design-patterns Go design-patterns Java sourcemaking - patterns and anti patterns. oodesign - patterns ca; | 2021 | 2021-09-22T22:10:51Z |
| github.com-sindresorhus-awesome_-_2022-01-24_22-26-30 | 😎 Awesome lists about all kinds of interesting topics My open source work is supported by the community Special thanks to: All your environment variables, in one place Stop struggling with scattered API keys, hacking together home-brewed tools, and avoiding access controls. Keep your team and servers in sync with Doppler. Your app, enterprise-ready. Start selling to enterprise customers with just a few lines of code. Add Single Sign-On (and more) in minutes instead of months. Strapi is the leading open-source headless CMS. It’s 100% JavaScript, fully customizable, and developer-first. Founded in 2018, OSS Capital is the first and only venture capital platform focused exclusively on supporting early-stage COSS (commercial open source) startup founders. Instant, shareable cloud PostgreSQL database Import any dataset in seconds, share with anyone with a click, try without signing up Dev environments built for the cloud Natively integrated with GitLab, GitHub, and Bitbucket, Gitpod au; | 2022 | 2022-01-25T02:20:23Z |
| github.com-sindresorhus-awesome_-_2023-10-24_23-57-20 | 😎 Awesome lists about all kinds of interesting topics Check out my latest app One Task Stop procrastination and get stuff done by focusing on one task at a time My open source work is supported by the community Special thanks to: Your app, enterprise-ready. Start selling to enterprise customers with just a few lines of code. Add Single Sign-On (and more) in minutes instead of months. Strapi is the leading open-source headless CMS. It’s 100% JavaScript, fully customizable, and developer-first. API to get search engine results with ease. API to get search engine results with ease. What is an awesome list? Contribution guide Creating a list Twitter Stickers & t-shirts Follow the Awesome Twitter account for updates on new list additions. Just type awesome.re to go here. Check out my apps and follow me on Twitter . Contents Platforms Programming Languages Front-End Development Back-End Development Computer Science Big Data Theory Books Editors Gaming Develop; | 2023 | 2023-10-25T11:07:00Z |
| CIA Reading Room cia-rdp85m00364r002304490085-0: MEETING WITH COMMUNITY PUBLIC AFFAIRS AND SECURITY OFFICERS ON 2 AUGUST 1983 TO DISCUSS LEAKS | Approved For Release 2008/04/18: CIA-RDP85M00364R002304490085-0 ~--, PAO 83-0004/6 25 July 1983 MEMORANDUM FOR: Director of Central Intelligence Director, Office of Security Lavon B. Strong Director, Public Affairs Office SUBJECT: Meeting with Community Public Affairs and Security Officers on 2 August 1983 to Discuss Leaks REFERENCES: a. 21 July 1983 Letter to Weinberger, Clark, Shultz Adelman re Cooperation to Prevent Unauthorized Disclosures b. 21 July 1983 Memorandum to Fourteen Community Public Affairs Officers, Same Subject 1. Action Requested: Authorize Public Affairs and the Office of Security to invite Community public affairs and security officers to meet with you on 2 August to discuss leaks. 2. Background: You have now sent letters (at left) to Community principals (Weinberger, Clark, Shultz, and Adelman) and to fourteen Community public affairs officers stating your concerns about leaks and describing your proposal to enlist their cooperation in notifying CIA of potentially; | 1983 | 2025-03-06T04:51:54Z |
| DTIC ADA416606: Mapping Critical DNA Sequence Elements Required for Amplification of erbB2 in Breast Cancer | To study the implications of replication initiator proteins to gene amplification, we characterized two proteins required for DNA replication (ORC and Mcm10). In an attempt to purify recombinant human ORC from insect cells infected with baculoviruses expressing HsORC subunits, we found ORC2, -3, -4, and -5 forms a core complex. Expression of ORC3N, which inhibits ORC2-ORC3 interaction, causes cell cycle arrest. Consistent with this result, ORC2-depletion by RNAi arrested cell cycle in G1 with low CDK activity. We propose that cells have a novel mechanism to ensure that CDK2 is activated only after enough pre-RC is formed. We also report that chromatin-loading of Mcm10 requires a functional pre-RC but is independent of Cdc7 activity. Mcm10 is required for chromatin-binding of Cdc45 and origin unwinding. By using EBV replication system in human cells, we report that overexpression of geminin inhibits replication of episome, but not that of host cell chromosome, suggesting a novel means b; | 2003 | 2018-05-14T06:25:43Z |
| DTIC ADA418662: Plant Estrogens: Effects on Cell Cycle Progression in Breast Cancer Cells | Epidemiological studies suggest that consumption of soy phytoestrgoens, such as genistein, is associated with a decrease risk of breast cancer. Genistein has been shown to exert chemopreventive and antiproliferative in animal models of cancer and breast cancer cells respectively. Accumulating evidence suggest that genistein is oxidatively metabolized by the cytochrome P45O (CYP45O) family of enzymes, and that its metabolism is specific to breast cancer cells but not nontransformed breast epithelial cells. We investigated the cellular association and effects of metabolism of genistein by CYP45O in T47D breast cancer cells and MCFlOA breast epithelial cells. These studies show that genistein exerts distinct effects on cell cycle progression that is due to its preferential cellular uptake and extent of its metabolism in T47D cells but not MCFlOA cells. Few studies describe the cell cycle effects of genistein on nontumorigenic breast epithelial cells. We compared the effects of genistein o; | 2003 | 2018-05-14T22:44:17Z |