| Title | Description | Published | Added |
| The three Jameses | | 1962 | 2025-05-22T16:21:27Z |
| Second international meeting of the international society for neurochemistry | | 1969 | 2025-09-08T04:17:18Z |
| Irradiation And The Nervous System | | 1963 | 2025-09-11T15:24:38Z |
| A Little Extra Effort | | 2007 | 2023-07-25T22:15:33Z |
| Research in physiology | | 1971 | 2025-09-08T00:26:07Z |
| Scientists In Organizations | | 1966 | 2025-09-11T18:21:23Z |
| De Racine Au Parthenon: Essais Sur La Litterature Et L'Art A L'age Classique | | 1977 | 2025-09-04T14:43:38Z |
| John Gay, his place in the eighteenth century | | 1938 | 2023-12-27T15:46:10Z |
| Marine molluscan genera of western north america | | 1963 | 2025-09-08T08:50:24Z |
| AD #3850 - Lyriq Now #2 in Cadillac Sales; GM Backs Off 1 Million EV Goal by 2025; Chinese Suppliers Race Into Mexico | - GM Backs Off 1 Million EV Goal by 2025- Lyriq Now #2 in Cadillac Sales- Forecasts BEVs to Hit 50% U.S. Sales by 2030- Cyberattack Cost Dealers $1 Billion- Lucid Claims Most Efficient EV- Stellantis Sues Another Supplier- Stellantis To Sell Recycled Oil- TotalEnergies to Install 3,000 Chargers in UK- With New A5 Audi Has No Coupe or Convertible- Chinese Suppliers Race Into Mexico- Suddenly EREVs Make A Lot of Sense | 2024 | 2024-11-16T17:25:08Z |
| Education and society in modern Germany | | 1949 | 2023-07-25T04:04:04Z |
| github.com-infracost-infracost_-_2022-03-24_17-01-48 | Cloud cost estimates for Terraform in pull requests💰📉 Love your cloud bill! Infracost shows cloud cost estimates for Terraform. It enables DevOps, SRE and engineers to see a cost breakdown and understand costs before making changes , either in the terminal or pull requests. This provides your team with a safety net as people can discuss costs as part of the workflow. Quick start 1. Install Infracost Assuming Terraform is already installed, get the latest Infracost release: macOS Homebrew: shbrew install infracost Linux/macOS manual download:```sh Downloads the CLI based on your OS/arch and puts it in /usr/local/bin curl -fsSL https://raw.githubusercontent.com/infracost/infracost/master/scripts/install.sh | sh``` Docker and Windows users see here . 2. Get API key Register for a free API key, which is used by the CLI to retrieve prices from our Cloud Pricing API, e.g. get prices for instance types. No cloud credentials or secrets are sent to the API and you can also self-host it. s; | 2022 | 2022-03-24T20:04:58Z |
| github.com-serverless-stack-serverless-stack_-_2022-02-07_09-07-40 | 💥 SST makes it easy to build serverless apps. Set breakpoints and test your functions locally. https://serverless-stack.com Serverless Stack (SST) is a framework that makes it easy to build serverless apps. It's an extension of AWS CDK and it features: A Live Lambda Development environment Support for setting breakpoints and debugging in VS Code Support for deploying to multiple environments and regions Higher-level constructs designed specifically for serverless apps Zero-config support for JS and TS (using esbuild ), Go, Python, C#, and F# Quick Start Create your first SST app. ```bash Create your app $ npx create-serverless-stack@latest my-sst-app$ cd my-sst-app Start Live Lambda Development $ npx sst start Deploy to prod $ npx sst deploy --stage prod``` Documentation SST docs SST examples Public roadmap Feature requests Contributing to SST Follow us on Twitter and subscribe to our newsletter for updates. About SST We think SST can make it dramatically easier to build serverless ; | 2022 | 2022-02-07T09:45:28Z |
| github.com-sottlmarek-DevSecOps_-_2022-01-14_09-42-59 | Ultimate DevSecOps library Ultimate DevSecOps library Contribution rules If you want to contribute to this library of knowledge please create proper PR (Pull Request) with description what you are adding following these set of rules: Clear description of PR (which tool, why, number of stars, maturity and topic) Keep it simple - Fill the description properly Fact over feelings or personal opinions Add source and follow the library style Avoid duplicits - one tool, one topic Try to make bigger updates then on tool link Currently open-source only Add only active projects Add only security tools Note: Currently this is an early version of the library. I recommend PR after first official release. DevSecOps library info: This library contains list of tools and methodologies accompanied with resources. The main goal is to provide to the engineers a guide through opensource DevSecOps tooling. This repository covers only cyber security in the cloud and the DevSecOps scope. Table of Contents Def; | 2022 | 2022-01-14T21:43:52Z |
| github.com-debezium-debezium_-_2021-12-10_11-35-09 | Change data capture for a variety of databases. Please log issues at https://issues.redhat.com/browse/DBZ. Copyright Debezium Authors.Licensed under the Apache License, Version 2.0 .The Antlr grammars within the debezium-ddl-parser module are licensed under the MIT License . English | Chinese | Japanese Debezium Debezium is an open source project that provides a low latency data streaming platform for change data capture (CDC). You setup and configure Debezium to monitor your databases, and then your applications consume events for each row-level change made to the database. Only committed changes are visible, so your application doesn't have to worry about transactions or changes that are rolled back. Debezium provides a single model of all change events, so your application does not have to worry about the intricacies of each kind of database management system. Additionally, since Debezium records the history of data changes in durable, replicated logs, your application can be stoppe; | 2021 | 2021-12-10T12:09:39Z |
| Cdk-mediated phosphorylation of the Kv?2 auxiliary subunit regulates Kv1 channel axonal targeting. | This article is from The Journal of Cell Biology , volume 192 . Abstract Phosphorylation of Kvβ2 releases Kv1 channels from microtubules to control their specific distribution at the axonal membrane. | 2011 | 2014-10-24T23:30:53Z |
| Are phylogenetic trees suitable for chemogenomics analyses of bioactivity data sets: the importance of shared active compounds and choosing a suitable data embedding method, as exemplified on Kinases. | This article is from Journal of Cheminformatics , volume 5 . Abstract Background: ‘Phylogenetic trees’ are commonly used for the analysis of chemogenomics datasets and to relate protein targets to each other, based on the (shared) bioactivities of their ligands. However, no real assessment as to the suitability of this representation has been performed yet in this area. We aimed to address this shortcoming in the current work, as exemplified by a kinase data set, given the importance of kinases in many diseases as well as the availability of large-scale datasets for analysis. In this work, we analyzed a dataset comprising 157 compounds, which have been tested at concentrations of 1 μM and 10 μM against a panel of 225 human protein kinases in full-matrix experiments, aiming to explain kinase promiscuity and selectivity against inhibitors. Compounds were described by chemical features, which were used to represent kinases (i.e. each kinase had an active set of features and an ina; | 2013 | 2014-10-28T15:40:11Z |
| Applied Stress Analysis | | 1967 | 2025-09-12T13:07:14Z |
| Islamic mathematical astronomy | 1 volume (various pagings) : 24 cm // Includes bibliographical references and indexes | 1993 | 2023-12-22T13:48:04Z |
| The leverage of labor : managing the Cortés haciendas in Tehuantepec, 1588-1688 | xxv, 245 p. ; 24 cm // Includes bibliographical references | 1989 | 2023-10-02T02:39:59Z |
| le bapteme des enfants | | 1948 | 2025-06-17T08:26:27Z |
| The fury out of time | | 1965 | 2023-08-09T07:48:05Z |
| Peterson's 4 year colleges, 2000 : the best advice, the best tools, the right guide | 3257 pages : 28 cm + // Includes index // Includes CD-ROM: College resources // System requirements: Microsoft Windows 3.1 or Windows 95/98; 25 MB free disk space; Windows-compatible sound card; 486/66 MHz or faster processor; 2X Multi-session CD-ROM drive; 8 MB RAM; SVGA 256 color graphics adapter and monitor; systems with 14.4 kbps or faster modem will enable CDK Internet connectivity | 1999 | 2022-10-04T23:03:24Z |
| Identification of Genes Interacting with rnt-1 Through Large-Scale RNAi Screening in Caenorhabditis elegans. | This article is from G3: Genes|Genomes|Genetics , volume 3 . Abstract Although many critical roles of the RUNX family proteins have already been identified, little attention has been given to how these proteins interact with other factors. Elucidating RUNX protein interactions will help extend our understanding of their roles in normal development and tumorigenesis. In this study, we performed large-scale RNAi screening to identify genes that genetically interact with rnt-1, the sole homolog of RUNX protein in the nematode Caenorhabditis elegans. To this end, we took advantage of the fact that C. elegans can survive a severe loss of RNT-1 function with only mild phenotypes, and we looked for genes that caused a synthetic phenotype in the rnt-1 mutant background. We identified seven genes, three of which (cdk-8, cic-1, and sur-2) are involved in transcription, two of which (pgp-2 and cct-5) are involved in stress response, and two of which (D2045.7 and W09D10.4) are involved in signalin; | 2013 | 2014-10-28T00:42:20Z |
| Periodic systems and their relation to the systematic analysis of molecular data | xxvi, 629 p., 8 p. of plates : 24 cm. + // Microfiches in pocket on front flyleaf // Includes bibliographies and index | 1989 | 2023-12-23T07:53:35Z |
| The saintmaker's Christmas Eve | | 1955 | 2023-08-11T09:11:11Z |
| The effects of modern mathematics | 436 p. : 25 cm // Bibliography: p. 415-436 | 1987 | 2023-12-22T13:33:59Z |
| ＦＲＩＧＨＴ ＮＩＧＨＴ ２[Visual Mixtape] | FRIGHT NIGHT 2 | Phonk Fridays ...𝔪𝔬𝔯𝔢 𝔱𝔥𝔞𝔫 𝔞 𝔰𝔢𝔮𝔲𝔢𝔩 𝔦𝔱'𝔰 𝔞 𝖘𝖈𝖗𝖊𝖆𝖒... FOR US, BY US VOL 666: Curated/mixed by iNFMS Full tape: https://soundcloud.com/iaminfms/fright-night-part-2 --------------------------------------------------------------------------------- T r a c k l i s t 00:20 : iNFMS - SPIT YO GAME [https://soundcloud.com/iaminfms] 02:13 : LORDKADO - MIDNIGHT SHOOTOUT w/ ABO [https://soundcloud.com/lordkado // https://soundcloud.com/mahatmaganja420] 04:37 : NIGHTINGALE - RED DROP [https://soundcloud.com/itsnightingale] 06:58 : otto22 - TAKE WHAT YOU WANT [https://soundcloud.com/otto22beats] 09:36 : WOBVSE - Hunter [https://soundcloud.com/wobase] 12:15 : DEISHIN - San [https://soundcloud.com/deishin] 14:40 : PXSSXSSXD - COLDEST [https://soundcloud.com/pxssxssxd] 18:05 : BEXFIELD - THOUGHT YOU KNEW [https://soundcloud.com/yaboibex] 20:15 : OG Marv - Water w/ RXIMV [https://soundcloud.com/ogmarv // https://; | 2019 | 2024-02-12T20:23:57Z |
| THE DON CHRONICLES: VOLUME THREE | The best of 2024 PHONK TAPES | iNFMS presents THE DON CHRONICLES: VOLUME THREE produced by iNFMS // Visuals by AnalogByNature respect this thing of ours... Volume 1: https://soundcloud.com/iaminfms/the-don-chronicles-vol-1 Volume 2: https://soundcloud.com/iaminfms/don-chronicles-2 Purchase: https://infms495.bandcamp.com/album/the-don-chronicles-volume-3 - dedicated to all the Fallen Soldiers, Hustlers, Macks, Pimps and Dons. Rest in power, gone but never forgotten. Shout Outs: STVSHBOI, Justillionaire, CDK, BADRADIO, SWAY, Flat Sprite, POSS PLAYA, SKINNYKID, Camyo, SHELTR. and LORDKADO - [00:00] H8 ME [03:29] TAKE NOTE$ [06:04] DOLLAZ N SENSE [08:16] HALF A MILL [10:42] WHY NOW? [13:53] PIMPIN HARD [16:46] INHALEXHALE [18:52] EARNERZ - linktr.ee/official_infms www.analogbynature.tv www.badradio.nz merch and stuff ; http://STORE.RYANCELSIUS.COM Official Spotify Playlist: https://spoti.fi/2V5Jzqr Join : https://bit.ly/2UIIw08 ▶▶ATTENTION ARTISTS AND PRODUCERS▶▶ ----Submissions: Ryan.Celsius@gmail.com ---100% F; | 2024 | 2024-08-11T21:38:21Z |
| linuxbrew cdk | linuxbrew cdk | | 2021-04-07T20:55:45Z |
| Psychology of Personal Adjustment Student's Introduction to Mental Hygience | | 1949 | 2025-09-10T00:53:02Z |
| IUMA: cdk Dashiell | Started like 6 months ago, now dominating the arts of sampling and sythetizing, I've decided to become a house dj, even got myself a couple of Gemini XL-400 II Turntables and a Gemini PMX 40 Mixer to start practicing for what I've greatly planned is my first presentation in a couple of years. That doesn't means that i've stopped producing! Actually I'm working with the Mirage corporation, a producing crew from Orlando, and another producer from Wisconsin, Da Corster. With both these influences I've learned a lot more and have applied it on this two new songs I got...Enjoy! :) | | 2012-05-18T17:23:07Z |
| odp-cdk-example | This is an archive of the project's publicly available source code and (where available) wiki repositories in bare git format, as well as related issues, pull requests and similar data from Github in JSON format, created on 2025-03-06. Project 'odp-cdk-example' from agency 'GSA', organization 'GSA'. Original project description: A serverless demonstration of the AWS CDK using Lambda, API Gateway, and Amazon S3 :rocket: | 2025 | 2025-03-12T18:53:16Z |
| yahoo-groups-2018-08-30T08-13-01Z-d5b6d2 | Yahoo! Groups API grab. Contains messages of the following groups: 08cecmcafriends 10thyec_documentations 10thyec_hrd 212sl_10 67_council_10-11 67_excom_10-11 aartedecompartilhar10 aborock accountantsworld advancedpca aggregator_game alcorp alyssamilanofansforever ambersdream api_2010 aulagelso_ceis aw88 basketball_ac beyondcircle bfpdatabasedevelopmentgroup bio11_lab_guzman blpholcimyli blueridgebirdexchange brandceo bsn2bnsginfo campusrecruitment cb7grupo252 cbhadventures ccih_aquiraz cdk_java cdpclub1011 cepe2000 clanrad_duty5 cmbe_ecrouves comissao_oab_se computer_lingap_1011 coolathawles cphic1994 csspvc daks_friends dapi_ii_2010a datasys_oracle_dba daytonactivitybagswap defensoriayplanmedico dhbn dimboola djjmatematika ec_azad_kermanshah_group economics_9a elenproj_ee energetica_iasi_1990 epdtributario-p04028 epmfam ex_smkbp feua2001 fkrkaceh fpik_umpgroup fumclovelandseniorhigh gangland2010 giusti12 houstonrubies h-v-v ibecbotucatu ibm379 ihrm_bertelsmann ipmiub iptn_2010 ipwija; | | 2018-08-30T08:13:09Z |
| die mikromorphologie der menschlichen zahnpulpa | | 1970 | 2025-09-04T05:39:32Z |
| Twinning And Diffusionless Transformations In Metals | | 1954 | 2025-09-12T13:34:58Z |
| THE EMPRESS FREDERICK | | 1956 | 2025-05-22T18:23:01Z |
| The life of Edward, Earl of Clarendon, Lord High Chancellor of England | | 1911 | 2024-01-12T08:38:15Z |
| Various Artists * 33,333 Steps To A Power Ov The Darkness Leagues | Various Artists * 33,333 Steps to a Power ov the Darkness Leagues (Disc one) 01. DJ R3V3R3ND MURD3R * !33,333 STEPS TO A POWER OV THE DARKNESS LEAGUES! 02. sOuL_sCientiSt * More Fiyah Mix 03. FIGURATIVE THEATRE * MEGAMARKET UTOPIA 04. Hel.Razor * Slave 2 The System 05. dreDDup * We Fuck Until Dawn 06. Moray Eel * Hellish Adoption 07. Cryogenic Implosion * Delete Humanity 08. The Apocryphal Order * Beyond The Depraved Scope Of Salvation 09. Come To My Rise * Play A Game 10. The Rinn * Voices 11. Crimson Blue * L.M.A. 12. Severed Fifth * Blackening 13. Spiritual Dissection * Across A Frozen Hell 14. Returning We Hear the Larks * Nascence in the Caverns of Mount Olympus 15. Cuntworm * Seduced By Damnation 16. Earslaughter * Roka Smrti (Disc two) 01. DiSloCaTed * Megazord 02. Cyber Snake * War 03. Circlerun * 13 04. Obsidian Shell * Misanthropia 05. Dawn Of Tears * Since They're Gone 06. Operación Mutante * Matanza 0; | 2013 | 2013-11-26T13:29:39Z |
| High-throughput screening with the Eimeria tenella CDC2-related kinase2/cyclin complex EtCRK2/EtCYC3a. | This article is from Microbiology , volume 158 . Abstract The poultry disease coccidiosis, caused by infection with Eimeria spp. apicomplexan parasites, is responsible for enormous economic losses to the global poultry industry. The rapid increase of resistance to therapeutic agents, as well as the expense of vaccination with live attenuated vaccines, requires the development of new effective treatments for coccidiosis. Because of their key regulatory function in the eukaryotic cell cycle, cyclin-dependent kinases (CDKs) are prominent drug targets. The Eimeria tenella CDC2-related kinase 2 (EtCRK2) is a validated drug target that can be activated in vitro by the CDK activator XlRINGO (Xenopus laevisrapid inducer of G2/M progression in oocytes). Bioinformatics analyses revealed four putative E. tenella cyclins (EtCYCs) that are closely related to cyclins found in the human apicomplexan parasite Plasmodium falciparum. EtCYC3a was cloned, expressed in Escherichia coli and purified in a co; | 2012 | 2014-10-28T23:41:32Z |
| The CDK regulators Cdh1 and Sic1 promote efficient usage of DNA replication origins to prevent chromosomal instability at a chromosome arm. | This article is from Nucleic Acids Research , volume 42 . Abstract Robustness and completion of DNA replication rely on redundant DNA replication origins. Reduced efficiency of origin licensing is proposed to contribute to chromosome instability in CDK-deregulated cell cycles, a frequent alteration in oncogenesis. However, the mechanism by which this instability occurs is largely unknown. Current models suggest that limited origin numbers would reduce fork density favouring chromosome rearrangements, but experimental support in CDK-deregulated cells is lacking. We have investigated the pattern of origin firing efficiency in budding yeast cells lacking the CDK regulators Cdh1 and Sic1. We show that each regulator is required for efficient origin activity, and that both cooperate non-redundantly. Notably, origins are differentially sensitive to CDK deregulation. Origin sensitivity is independent on normal origin efficiency, firing timing or chromosomal location. Interestingly, at a chrom; | 2014 | 2014-10-21T00:10:14Z |
| AD #3841 - Rivian Hits Paydirt With Massive VW Investment; Revealed The Last Pontiac Concept Car; CATL Flies 4-Ton Electric Plane | - Rivian Hits Paydirt With Massive VW Investment- Rivian Hints At 5 Future Models- Tesla Drops Under 50% U.S. EV Market Share- CDK Cyberattack Lasts Longer Than Expected- U.S. June Sales At 16.2 Million SAAR- Revealed The Last Pontiac Concept Car- GM-SAIC Use Robots for Car Testing- Bentley Unleashes Most Powerful Model Ever- CATL Flies 4-Ton Electric Plane | 2024 | 2024-11-16T18:41:55Z |
| Indole-3-Carbinol Inhibits Nasopharyngeal Carcinoma Growth through Cell Cycle Arrest In Vivo and In Vitro. | This article is from PLoS ONE , volume 8 . Abstract Nasopharyngeal carcinoma is a common malignant tumor in the head and neck. Because of frequent recurrence and distant metastasis which are the main causes of death, better treatment is needed. Indole-3-carbinol (I3C), a natural phytochemical found in the vegetables of the cruciferous family, shows anticancer effect through various signal pathways. I3C induces G1 arrest in NPC cell line with downregulation of cell cycle-related proteins, such as CDK4, CDK6, cyclin D1 and pRb. In vivo, nude mice receiving I3C protectively or therapeutically exhibited smaller tumors than control group after they were inoculated with nasopharyngeal carcinoma cells. The expression of CDK4, CDK6, cyclin D1 and pRb in preventive treatment group and drug treatment group both decreased compared with the control group. We conclude that I3C can inhibit the growth of NPC in vitro and in vivo by suppressing the expression of CDK and cyclin families. The drug was s; | 2013 | 2014-10-27T03:53:00Z |
| Applications of the InChI in cheminformatics with the CDK and Bioclipse. | This article is from Journal of Cheminformatics , volume 5 . Abstract Background: The InChI algorithms are written in C++ and not available as Java library. Integration into software written in Java therefore requires a bridge between C and Java libraries, provided by the Java Native Interface (JNI) technology. Results: We here describe how the InChI library is used in the Bioclipse workbench and the Chemistry Development Kit (CDK) cheminformatics library. To make this possible, a JNI bridge to the InChI library was developed, JNI-InChI, allowing Java software to access the InChI algorithms. By using this bridge, the CDK project packages the InChI binaries in a module and offers easy access from Java using the CDK API. The Bioclipse project packages and offers InChI as a dynamic OSGi bundle that can easily be used by any OSGi-compliant software, in addition to the regular Java Archive and Maven bundles. Bioclipse itself uses the InChI as a key component and calculates it on the fly whe; | 2013 | 2014-10-27T02:14:25Z |
| Regulation of T Cell Differentiation and Alloimmunity by the Cyclin-Dependent Kinase Inhibitor p18ink4c. | This article is from PLoS ONE , volume 9 . Abstract Cellular proliferation in response to mitogenic stimuli is negatively regulated by the Cip/Kip and the Ink4 families of cyclin-dependent kinase (CDK) inhibitors. Several of these proteins are elevated in anergic T cells, suggesting a potential role in the induction or maintenance of tolerance. Our previous studies showed that p27kip1 is required for the induction of T cell anergy and transplantation tolerance by costimulatory blockade, but a role for Ink4 proteins in these processes has not been established. Here we show that CD4+ T cells from mice genetically deficient for p18ink4c divide more rapidly than wild-type cells in response to antigenic, costimulatory and growth factor signals. However, this gain of proliferative function was accompanied by a moderate increase in the rate of cell death, and was accompanied by an overall defect in the generation of alloreactive IFNγ-producing effector cells. Consistent with this, p18ink4c-d; | 2014 | 2014-10-24T00:58:58Z |
| Kirkland City Council Regular Meeting Summary – January 2, 2024 | # Kirkland City Council Regular Meeting – January 2, 2024 The Kirkland City Council held a hybrid regular meeting on Tuesday, January 2, 2024, from 7:30 p.m. to 10:15 p.m. The council administered oaths to newly elected members, selected Mayor Kelli Curtis and Deputy Mayor Jay Arnold, approved a consent calendar, adopted the 2023-2028 Transportation Improvement Program, received a briefing on a Redmond paramedic co-location agreement, reviewed 2024 committee assignments, and discussed next steps after the November 2023 pool ballot measure failed. All seven councilmembers were present initially; Councilmember Jon Pascal left at 7:45 p.m. due to illness and was excused for the remainder of the meeting. ### Oaths, Mayor and Deputy Mayor Selection - Municipal Court Judge John Olson administered oaths of office to re-elected Councilmembers Kelli Curtis and Amy Falcone and newly elected Councilmember John Tymczyszyn. - The council voted 7-0 to select Kelli Curtis as Mayor and 7-0 to select; | 2024 | 2026-09-07T18:44:16Z |
| Overbrenging archieven Rijkstaken CdK naar het Historisch Centrum Overijssel | | 2015 | 2018-03-12T22:24:05Z |
| Pride Flag Raised in Silver Spring | Montgomery County celebrates Pride Month Music provided by Manhattan Production Music. Montgomery Community Media (MCM) is the only independent, nonprofit organization providing access to public media (public access television and broadband media) directly to Montgomery County’s over one million residents as well as the resident non-profit organizations, associations and businesses. We engage, educate and empower individuals and the community to express diverse viewpoints. Stay up to date on news in Montgomery County! » Subscribe to MCM here: https://goo.gl/fi9sqQ » Check out MCM for more: https://www.mymcmedia.org Follow MCM on these platforms! » Facebook: https://goo.gl/89YTJN » Twitter: https://twitter.com/mymcmedia » Instagram: https://goo.gl/BkuFnD For more Montgomery County, Maryland residents can tune to Comcast channels 21 and 995 | 2022 | 2022-06-02T01:32:19Z |
| Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4. | This article is from PLoS Genetics , volume 10 . Abstract DNA double-strand breaks (DSBs) can be repaired by one of two major pathways—non-homologous end-joining (NHEJ) and homologous recombination (HR)—depending on whether cells are in G1 or S/G2 phase, respectively. However, the mechanisms of DSB repair during M phase remain largely unclear. In this study, we demonstrate that transient treatment of M-phase cells with the chemotherapeutic topoisomerase inhibitor etoposide induced DSBs that were often associated with anaphase bridge formation and genome instability such as dicentric chromosomes. Although most of the DSBs were carried over into the next G1 phase, some were repaired during M phase. Both NHEJ and HR, in particular NHEJ, promoted anaphase-bridge formation, suggesting that these repair pathways can induce genome instability during M phase. On the other hand, C-terminal-binding protein interacting protein (CtIP) suppressed anaphase bridge formation, implying that CtIP fu; | 2014 | 2014-10-06T07:32:29Z |
| DTIC ADA429736: TGF-Beta Resistance in Breast Cancer | Transforming growth factor-Beta (TGF-Beta) potently inhibits cell proliferation by causing cell cycle arrest at the Gl phase. Smad proteins mediate the TGF-Beta antiproliferative responses by regulating the expression of several cell cycle components. We have recently discovered that Smad3, a key Smad protein for the TGF-Beta antiproliferative responses, is a major physiological substrate for cyclin-dependent kinases CDK4 and cDK2. Except for the Rb family members, Smad3 is the only cDK4 substrate demonstrated so far. CDK phosphorylation of Smad3 inhibits its transcriptional activity and antiproliferative function. Since cyclin Dl that activates CDK4 and the related cDK6 is amplified or overexpressed in a high percentage of human breast cancers, we examined whether Smad3 is highly phosphorylated by CDK in breast cancers. Using primary human breast cancer tissue microarrays and a phosphopeptide antibody against a CDK phosphorylation site in Smad3, we have found that overexpression of cy; | 2004 | 2018-05-22T14:32:34Z |