| Title | Description | Published | Added |
| CDK Construction Co., Inc. v. Parkway C&A, LP | This item represents a case in PACER, the U.S. Government's website for federal case data. If you wish to see the entire case, please consult PACER directly. | | 2025-05-09T09:18:03Z |
| Het Utrechts Archief 807112 | Het Utrechts Archief catalog number: 807112 Description (Dutch): Afbeelding van het electrische treinstel nr. 461 (mat. 1936) van de N.S.N.B. Het treinstel 461 was een electrisch drierijtuigen-treinstel dat na de oorlog is samengesteld uit bakken van diverse treinstellen (Bk 607, C 613 en CDk 610). Het treinstel werd voornamelijk gebruikt voor het vervoer van het Concertgebouworkest. | 1960 | 2021-02-03T04:46:45Z |
| Scientists In Organizations | | 1966 | 2025-09-11T18:21:23Z |
| Timely Activation of Budding Yeast APCCdh1 Involves Degradation of Its Inhibitor, Acm1, by an Unconventional Proteolytic Mechanism. | This article is from PLoS ONE , volume 9 . Abstract Regulated proteolysis mediated by the ubiquitin proteasome system is a fundamental and essential feature of the eukaryotic cell division cycle. Most proteins with cell cycle-regulated stability are targeted for degradation by one of two related ubiquitin ligases, the Skp1-cullin-F box protein (SCF) complex or the anaphase-promoting complex (APC). Here we describe an unconventional cell cycle-regulated proteolytic mechanism that acts on the Acm1 protein, an inhibitor of the APC activator Cdh1 in budding yeast. Although Acm1 can be recognized as a substrate by the Cdc20-activated APC (APCCdc20) in anaphase, APCCdc20 is neither necessary nor sufficient for complete Acm1 degradation at the end of mitosis. An APC-independent, but 26S proteasome-dependent, mechanism is sufficient for complete Acm1 clearance from late mitotic and G1 cells. Surprisingly, this mechanism appears distinct from the canonical ubiquitin targeting pathway, exhibitin; | 2014 | 2014-10-11T23:56:58Z |
| ginerbreadman | ginerbreadman | | 2022-11-15T19:45:05Z |
| Plant WEE1 kinase is cell cycle regulated and removed at mitosis via the 26S proteasome machinery. | This article is from Journal of Experimental Botany , volume 64 . Abstract In yeasts and animals, premature entry into mitosis is prevented by the inhibitory phosphorylation of cyclin-dependent kinase (CDK) by WEE1 kinase, and, at mitosis, WEE1 protein is removed through the action of the 26S proteasome. Although in higher plants WEE1 function has been confirmed in the DNA replication checkpoint, Arabidopsis wee1 insertion mutants grow normally, and a role for the protein in the G2/M transition during an unperturbed plant cell cycle is yet to be confirmed. Here data are presented showing that the inhibitory effect of WEE1 on CDK activity in tobacco BY-2 cell cultures is cell cycle regulated independently of the DNA replication checkpoint: it is high during S-phase but drops as cells traverse G2 and enter mitosis. To investigate this mechanism further, a yeast two-hybrid screen was undertaken to identify proteins interacting with Arabidopsis WEE1. Three F-box proteins and a subunit of t; | 2013 | 2014-10-23T22:24:00Z |
| CIA Reading Room cia-rdp80-00810a000600660006-7: RAILROAD COAL STOCKS AND STATUS OF RAILROAD CARS | 25X1 ApFrP 1 1t aXe amn~/j : CIA-RDP80-0081 OA0006006600 6-7 25I I CENTRAL I 25XIEPORT NO, L INFORMATION REPORT CD NO. COUNTRY Iket Cary SUBJECT Rail.rocd Cool Stockal and Statu?s of Railrord Coro 25X1 PLACE .CQU1'? ECG )ATE CF INFO. T a, OOCU!,-V 3T COOTASNS Ili POP.D1IT4OF1 A"ACTINOTHE NATIONAL lib FEN1R P.'' O8 T141 UHi 1ZD STATES, tIT:lmf1 THE HBANIUIG OF TITLE 28. SFr TIGHS 703 I! i -D 794. 0: THE U. S. CODE, A.3 ASSEH050. IT5 TAAHSIiISSION ~)4I Rd1:EL? I' OI> OF 415 C0RT01470 TD O:7 RECEIPT ay AH UNAUTHOTIIZED PE100.0 f TS ":' mUl GIT D DY AV THE REPRODU70-t OP ':'H1S FORA IS PR-IuIIIITED. 25X1 25X1 Coal stocks and ;onsurr Lion of coal :I: ca o pe ua ~,.- l' ti Date in Hard Coal Raw E oT>r Jam-ary Coal `iota::. 21 49,131 17,280 Stocks 22 48,028 17,191 46,668 18,043 }3 } 25 25X1 DA FE 16)),c-TW 9 April 3.953 NO. OF Fii=GES NJO.. OF G--'1 CLS, {LUSTED E..iL(1'r!} #3:+L1: `ENT y~ y.f REPORT NO. THIS IS UNEJ''ELUAliTD !1[,,i'OP Q 61\14Oii ;+ c$Z? ? ttc 3~ 76,6ci 78,0313 76,724, 2%::,; | 1953 | 2024-02-24T10:38:21Z |
| github.com-lloesche-valheim-server-docker_-_2021-03-15_23-08-06 | Valheim dedicated gameserver with automatic update, World backup and ValheimPlus mod support lloesche/valheim-server Docker image Valheim Server in a Docker Container (with ValheimPlus support) Table of contents Basic Docker Usage Environment Variables Log filters Event hooks Event hook examples Install extra packages Copy backups to another location Delay restarts by 1 minute and notify on Discord ValheimPlus config from Environment Variables System requirements Deployment Deploying with Docker and systemd Deploying with docker-compose Deploying to Kubernetes Deploying to AWS ECS Updates Backups Finding Your Server In-game Steam Server Browser Steam Server Favorites & LAN Play Admin Commands Supervisor Supervisor API Status web server ValheimPlus Updates Configuration Server data rate Disable server password Changing startup CMD in Portainer Synology Help First install Updating the container image to the latest version Error after download of new container image Basic Docker Usage The; | 2021 | 2021-03-16T07:10:56Z |
| yahoo-groups-2018-06-30T23-49-20Z-213b6b | Yahoo! Groups API grab. Contains messages of the following groups: abass_t92 adaevi adeala agec424group almostfamous-bvds apurv_heavymetal arch_ugm_05 asd-trainees-0809 avrilavigneavlav aw_groups backlanebizneskl baeeunice bazket_sport besmart_group biotechresearchers bscs_1b_prog_cdk bsn_4a_frieslovers c3_noticeboard cagarencuclillas camobutts ccsflitjournal cfne_22008 chatters cheap-pet-supplies cicstcollege cisco_ghana clubfiatecuador cmpe274_business_intelligence_fall2008 coa08_iiu conexaoamsterda coolfriends_ngp cool_raj1989_hot copi_nijmegen cosbuc2008_12r1 cpa-world csc_8 cscst-argaocampusmis-ict darla_fux52a_livecams darvazeh dc_metro_angels dhrumil88 digitaldial_diversidial_online ds112grouppapergk dyala eca_maniez efbalicobacoba entreprisemichite fierydemon2008 filmstadensickla fina6335_uh2008 flat4677 fuad_online ga-fesc garciamendozafamilytree gay60plus gcet_cs2012 gdc-organic gherti-entertainment ghp_group gkm_2k8 grandmas-recipe-box grupoutp113 gw78 het_tien_roi_89 hottes; | | 2018-06-30T23:49:27Z |
| CIA Reading Room cia-rdp91-00929r000100090020-0: FR:KURTA G. TO:KUGAKOV M.M. | Retaxmion Phenomew for Attenwalm of Sxvdin Ductop by G. K=txp.GERMM, per, A~M Vol )as 1961, pp 112-117.NASA TT P-9277Sci-Mectrmay 65U.S. GOVERNMENT ONLY28(4190mTransplantation of Himan Carcitwom.9 by Raw Kurtzabn,10 pp?)=WAN, perp Ki. usch.., Vol Uls, 1926o pp 1166-1168.inScientific - Medicine CTS/DIX // .2 P401Proceocs lor Yonufactur-ling a Cb--ese ?rwuct of GrGratGd Hl&~i-Fat Chaese, by 2. 24 Tlxalsmaa,W, J. Kurt--lial3,, 6 pp.amm, potperrit 949,6i6i 30 Aug -1956.sLA 6o-149aI f- -1 -7,SciROSTE.Tmals B. 1.KURUKLISP G. A.Stanki I instrument# Vol. 17, xo. 6, 11figures wo 3 tableav 2600 words- 1946.Deep Dry Cyaniding of Cutting- Toole.Brutcher Trans, Order No* 1914s, $4-55amom Lai to latruall"Is 3*MQWbt,-Nw 73cateshoWalow contsm As foblize 40""*1. rA* 4UNft Of & IPYT*V"* &*SUQW(""I) ft sbo AOL".' coutaft of the sx*itStan a" on ow ant" Tore"sumaby T. gulmas *t RuJAPAUMS, Fifirs MUM TakwLELS it Val LisU64v pp 463oMaw MIN X0scl - S/wMay 66The !~!chanlce c-f Beending of the SbRP.-, of incUne; | 1983 | 2026-03-25T15:12:02Z |
| CIA Reading Room cia-rdp96-00788r000700130014-7: SPOT REPORT | F t'R. C EFIC,\L USE ONL y 4########### PEt IT AGU i IELFCG?tilLNICAT IONS CEN TEI PTTEZYL , RUavfMFDA01315 0252257 IJR i TY/KDUT INE r' 1 252BUOZ JAN 82 h-,vj C(JRI:SAINiSC(7~ MI ()ET FT LEWIS WA //LAGPA-C-LE// TL CDK SPECIAL 'PINS CET '.-1 NEADE MD //IASC// +U/_L;;~IC,E FT M CACE D SL1// CC;z JSAIT A C AHS Vi\ X-T,\-t?,// l-t0 DA WASF-GC //DAM, -CIC/FIO// 11,LLL'i FT I"NSCIJ' :\HS VA // i. L PS ('SF) SFFAN CA //IAGPA-C-OP// C'_ E~ S c r- I iU F JUC NU N Iuh,T AC T I (iNN REL;U IR EC T I ~, ,:) L i7 = Z r ; ,. l ( OT kE PnF r i . ( F'-jLi)) K1J?JAP0I iNB CF 13L JAMES DEL LEN, ASS FEPGPTED F3Y GERALDINE ILS ("\? A':(,RY TARASFWICH9 :1N() R5 ARA KiJPIK P1,TTEN, KENT, WASHINGTON, ['-;LaE (/. b) 631_.68G6 (CFFICEI- 631-?81)18 (H0MEI, F.4 RA S" C3 ( rS r.:vcF =". (I i?`iITI,AL SP ur r,Ei-~:ir,T9 SAD, 2U24CUZ JAN 82. L~m 2, (U) G I.LNH tFPC1RI 3 (a l (FLUUI i N! 7.1 JA,' 82 , FLMID ,AS 0:KrHF. VISIilNS CONCERNING 10TH` PLACE J~=5C1 | 1982 | 2023-06-26T04:57:16Z |
| Het Utrechts Archief 846446 | Het Utrechts Archief catalog number: 846446 Description (Dutch): Interieur van het electrische treinstel nr. 461 (mat. 1936) van de N.S.N.B. Het treinstel 461 was een electrisch drierijtuigen-treinstel dat na de oorlog is samengesteld uit bakken van diverse treinstellen (Bk 607, C 613 en CDk 610). Het treinstel werd voornamelijk gebruikt voor het vervoer van het Concertgebouworkest. | 1960 | 2021-02-27T20:18:20Z |
| Crazy Bytes 4 [REPACKED] | First, I'd like to thank the original uploader for this archive which contains some rare gems. Like some others, I had difficulty accessing the files easily. This isn't intended to stop on anyone's toes, but rather make it more readily accessible. If you are seeking the original, untouched archive go here: https://archive.org/details/CrazyBytes044CD If you simply want the software, continue on. The original text encoding on the password was difficult to work with. Now, everything has been zipped without passwords and all original file timestamps have been preserved. It is not necessary to run the menu or other included software to access the files. There are 6 .RAR files which will not fully extract because of CRC errors. While they can partially extract, they're likely corrupted or missing files. These corrupted RARs are included for consistency. The rars are in the zip of their respective folders. Unzip to see find the rar and it's password. If you'd like to try extracting the ; | | 2022-01-21T22:02:32Z |
| Differential roles of Smad2 and Smad3 in the regulation of TGF-?1-mediated growth inhibition and cell migration in pancreatic ductal adenocarcinoma cells: control by Rac1. | This article is from Molecular Cancer , volume 10 . Abstract Background: Progression of pancreatic ductal adenocarcinoma (PDAC) is largely the result of genetic and/or epigenetic alterations in the transforming growth factor-beta (TGF-β)/Smad signalling pathway, eventually resulting in loss of TGF-β-mediated growth arrest and an increase in cellular migration, invasion, and metastasis. These cellular responses to TGF-β are mediated solely or partially through the canonical Smad signalling pathway which commences with activation of receptor-regulated Smads (R-Smads) Smad2 and Smad3 by the TGF-β type I receptor. However, little is known on the relative contribution of each R-Smad, the possible existence of functional antagonism, or the crosstalk with other signalling pathways in the control of TGF-β1-induced growth inhibition and cell migration. Using genetic and pharmacologic approaches we have inhibited in PDAC cells endogenous Smad2 and Smad3, as well as a potential regulator, th; | 2011 | 2014-10-29T05:01:17Z |
| Cyclin D1 inhibits whereas c-Myc enhances the cytotoxicity of cisplatin in mouse pancreatic cancer cells via regulation of several members of the NF-?B and Bcl-2 families. | This article is from Journal of Carcinogenesis , volume 10 . Abstract Background:: Cisplatin (CDDP) is a drug used for treatment of many types of malignancy but pancreatic cancer is relatively resistant to it. This study aims to determine whether and how cyclin D1 (D1) and c-Myc influence the response of pancreatic cancer cells to CDDP. Materials and Methods:: Ela-mycPT mouse pancreatic cancer cells were transfected with D1 or c-myc cDNA and treated with CDDP alone or together with NPCD, an inhibitor of cyclin dependent ckinase (CDK) 4 and 6. Reverse transcription followed by polymerase chain reaction (RT-PCR) and western blot assays were used to determine the mRNA and protein levels of interested genes. Cell viability was determined using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results:: Treatment of Ela-mycPT1 cells with CDDP caused an increase in c-myc expression but a slightly latent decrease in D1 expression, whereas D1 and c-Myc proteins repres; | 2011 | 2014-10-28T18:52:34Z |
| Overtures Of Biology | | 1964 | 2025-09-11T12:55:51Z |
| Zds1 regulates PP2ACdc55 activity and Cdc14 activation during mitotic exit through its Zds_C motif. | This article is from Journal of Cell Science , volume 125 . Abstract At anaphase onset, highly active mitotic cyclin-dependent kinase (Cdk) is inactivated to promote exit from mitosis and completion of cytokinesis. The budding yeast Cdc14p phosphatase is a key mitotic regulator that counteracts cyclin-dependent kinase (Cdk) activity during mitotic exit. Separase, together with Zds1p, promotes the downregulation of the protein phosphatase 2A in conjunction with its Cdc55p regulatory subunit (PP2ACdc55) in early anaphase, enabling accumulation of phosphorylated forms of Net1p and release of Cdc14p from the nucleolus. Here we show that the C-terminal domain of Zds1p, called the Zds_C motif, is required for Zds1-induced release of Cdc14p, and the N-terminal domain of the protein might be involved in regulating this activity. More interestingly, Zds1p physically interacts with Cdc55p, and regulates its localization through the Zds_C motif. Nevertheless, expression of the Zds_C motif at endo; | 2012 | 2014-10-28T00:57:58Z |
| the valaue theory of karl marx | | 1976 | 2025-05-22T13:48:47Z |
| Ep 10 Part 1: Kristine Bunch's Nightmare | In part 1 of this episode, you'll hear about one woman's nightmare come true. In 1995, 21-year-old Kristine Bunch was abruptly awoken in the early morning hours to discover that her house was on fire. After escaping the flames, she watched in horror as firefighters were unable to save her 3-year-old son who was still trapped inside. Within hours of being released from the hospital, she discovered that detectives had a theory about the fire - someone had started the fire on purpose, and their main suspect was Kristine. A special thank you to Kristine Bunch for helping me tell her story.Please consider supporting Court Junkie with as little as $3 a month via Patreon.com/CourtJunkie. Help support Court Junkie with $6 a month and get access to bonus monthly episodes.Follow me on Twitter @CourtJunkiePod or Instagram at CourtJunkie. Music featured in this episode: Like Music (cdk Mix) by cdk (c) copyright 2015 Licensed under a Creative Commons Attribution Share-Alike (3.0) license. htt; | 2016 | 2023-07-08T12:43:39Z |
| CIA Reading Room cia-rdp91-00403r000200380008-6: FR:IZSO L TO:BAGER G | Traffic Organization and Preparation of Tim Tableofor Pusenger Shippliig on the Balaton.MMGARIANI perk Kozlekedese KozIony) 24 may 1970.,PP 384-387.DTA LN 1328-70Ju1Y 71The Plans and Tasks of Mobart for the Year 1970.RMARIM., per. KozIekedesi Kbzlony, No 16,19 April 1970, p 285.DTA rX 114g-7oJ'AlY 71Trends In Transportmbion and Postal Output Devicing1969.MMGMIM~ perk Kozelekedsi lCozIony, No 153April 12, 1970, p~ M7-2',11.DIA LN 1148-70May 71The Twerity-Five Years of Hungarian Shipping.UMMIM, per, I-a-ziekedesi KoziMp .4 April 1970sPp 248-250-DiA Lw 1147-70State PAIUVWU# IM Task* MftbWobyWay &*Wlo 9 pp.HUWARW Paogo 90 IYAOr 0ins 50417RF,NungaryF400&F ?0 407j,346V^aq;mBow&r 70 4020445NW& 7111 lans for I~ailroad Containerization wotexi.by Laszlo Izao, k~yorgy ionyrd, 9 pp,10WARIAW, por, Xozlq)cedAk 1~q pnv, lWapust,30 I'~ov 1969. pp W~Z~d- L4 _-i ei~s 49688U~ImngaryBconi-eb 70 40o,6o,6-laticnal aid i iiaij)our L,; 40I )ILL.14lUji"'It"L.'j, ~ILEi2!L~ 17 ~.;,),vo, 1'J69Ilil.,\ 1~~ U48-69(D; | 1983 | 2026-03-31T13:43:14Z |
| CIA Reading Room cia-rdp86b00985r000100080009-5: LETTER TO MS. MINNIE REED DIRECTOR OF CAREER SERVICES PRINCETON UNIVERSITY | Approved For lease 2005/07/14: CIA-RDP86B00985~00100080009-5 CENTRAL [iVTl;!_LIGENCE AGENCY' WASHINGTON, A. C. 20505 PUBLIC AFFAIRS 3'hone: (703) 351-767!5 6 November 1981 Ms. Minnie Reed Director of Ca.re~er Services Clio Hall Princeton University Princeton, New Jersey 08544 -Dear Pis . Reed In connectiion with the forthcoming visit of CIA Personnel STAT Officer to Princeton, you have inquired as to the Agency's po icy w~ regard to hiring homosexuals. The CIA selects its employees on the basis ofi professional qualifications and aptitudes alone. Agency employees are entrusted with access to highly sensitive classified information relating to the national security. Consequently, before employment procedures are completed and an employee enters on duty the employee must receive a security clearance. f-or this purpose the employee must be deter-- - mined to be of unquestioned loyalty and.gaod character. Moreover, the employee must be found not likely to?be susceptible to techniques known; | 1981 | 2024-05-13T17:20:36Z |
| CIA Reading Room cia-rdp91-00403r000200500046-0: FR:BARBIERI R TO:BARDENHEUER P | Research on Vackarmoderle Reaction.Note 11. On the Maolianism of theYormatiou of lutovuul S-,alfur inPolythionic Acid9l, by Ro Barbieri*ITALIANp perlVvI 920, 1%2* 0-6?5NTO 72-12316-07BJuly 72Studleo on the Weakenrodar Reaotion.Note 1. Thiaoulphurie Acids as ProbableIntermediate Produatep by Re Barbieri.ITALIAIT, parp Ricerea Scientifica.Far&a 11, Hendleonti.Vol 2j No 1,, 19620 pp 62-67073July 72investigations of the Reaction H2StSO2Analytical Procedures for Separatingand Breaking Down Maercquantities ofNfur Componanto in Research WithS , by Ro Barbieri*ITALIAN# porp ifigalVol 30, 1960l pp 1509-1517UTO 72-11838-07BJune 72.191 ( [erawl. M2911AVIM, Gliuscme). ami" 63-129098eHiuf['MbaMj. 1. Barbieri, R*AtULYSIS OF ORGANOMETALUC CONIOUNDS. 17. Taghavint, G.DITIMONATES OF RjPbCI AND R2PbC]- f19621.10p.K-H50 K-H121 MOdf M. BaJilgo, U.K -H - 1117IV1aromer,r1 1 - a-V. Kresge-Hooker7YRm Of [U] U, y) 1960, Science Mraryv. 30, no. 12, p. 1963-1968. Associatca, Detroit, Mich.DESCMMRS: 0=1cal andysi; | 1983 | 2026-03-26T07:30:01Z |
| cyyo oyousym – doxzmzytr yoizc jyioyzu | cynmso ntyniyyzd kyjsq sgqkd iqsukz lncnjz yo ioxzmzyt. dopny-tn gmykd kr zsdk, yz n cdk yy sqkod, aingyn lirgdk ln tycyyzq. c gsmqm — moxgzipo, z yzpopzc qpoxqqdzc pokyyu. vzptn yy lnsapojyt tyxy, pqso aiysqyt agymyqtu, ctnxr snatzcu acoatud. vzptn yy lnsapojyt tyxy gymyru lqtu, gysu jzxyu yy apoxpo. fniupn g apoxpy npmqjoqdzy syidtad yo snxmrc z xirc, g syuatgztyiuynatz jy gad mnmoxsn ainjyyy. | | 2024-07-06T12:14:46Z |
| Roscovitine confers tumor suppressive effect on therapy-resistant breast tumor cells. | This article is from Breast Cancer Research : BCR , volume 13 . Abstract Introduction: Current clinical strategies for treating hormonal breast cancer involve the use of anti-estrogens that block estrogen receptor (ER)α functions and aromatase inhibitors that decrease local and systemic estrogen production. Both of these strategies improve outcomes for ERα-positive breast cancer patients, however, development of therapy resistance remains a major clinical problem. Divergent molecular pathways have been described for this resistant phenotype and interestingly, the majority of downstream events in these resistance pathways converge upon the modulation of cell cycle regulatory proteins including aberrant activation of cyclin dependent kinase 2 (CDK2). In this study, we examined whether the CDK inhibitor roscovitine confers a tumor suppressive effect on therapy-resistant breast epithelial cells. Methods: Using various in vitro and in vivo assays, we tested the effect of roscovitine on th; | 2011 | 2014-10-28T11:55:39Z |
| Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers. | This article is from eLife , volume 3 . Abstract During M phase, Endosulfine (Endos) family proteins are phosphorylated by Greatwall kinase (Gwl), and the resultant pEndos inhibits the phosphatase PP2A-B55, which would otherwise prematurely reverse many CDK-driven phosphorylations. We show here that PP2A-B55 is the enzyme responsible for dephosphorylating pEndos during M phase exit. The kinetic parameters for PP2A-B55’s action on pEndos are orders of magnitude lower than those for CDK-phosphorylated substrates, suggesting a simple model for PP2A-B55 regulation that we call inhibition by unfair competition. As the name suggests, during M phase PP2A-B55’s attention is diverted to pEndos, which binds much more avidly and is dephosphorylated more slowly than other substrates. When Gwl is inactivated during the M phase-to-interphase transition, the dynamic balance changes: pEndos dephosphorylated by PP2A-B55 cannot be replaced, so the phosphatase can refocus its attention on CDK-phospho; | 2014 | 2014-10-23T20:21:36Z |
| Topology of C. W. Complexes | | 1969 | 2023-12-22T18:35:46Z |
| CIA Reading Room cia-rdp83m00914r002800040028-2: REQUEST FOR INTERVIEW - JOSEPH FROMM, U.S. NEWS & WORLD REPORT | Approved For Relgrase 2007/03123: CIA-RDP83M00914R(~~C~4f~'f OEXA 82-272 MEMORANDUM FOR: Director of Central Intelligence FROM: erector, Office of External Affairs STAT SUBJECT: Request for Interview - Joseph Fromm, U.S. News & World Report 1. Action Requested: Decline an on-the-record interview with Joseph Fromm, U.S. News & World Report. 2. Background: Fromm's telephoned request is based on his impression that you would like to get your views accurately on the public record. He offers a recorded interview with a pre-publication screening opportunity. The subject would be the role of .intelligence and your role in improving it. Joseph Fromm and other reporters met with you at a breakfast meeting at Headquarters in Rpril 1981. 3. Staff Position: Too frequent on-the-record interviews are not consistent with the current low-profile policy. One leads to another. Your recent interview with David Kahn should suffice for awhile, unless you have some special reason for doing another. The DDCI; | 1982 | 2025-02-22T17:14:43Z |
| Pterostilbene Simultaneously Induced G0/G1-Phase Arrest and MAPK-Mediated Mitochondrial-Derived Apoptosis in Human Acute Myeloid Leukemia Cell Lines. | This article is from PLoS ONE , volume 9 . Abstract Background: Pterostilbene (PTER) is a dimethylated analog of the phenolic phytoalexin, resveratrol, with higher anticancer activity in various tumors. Herein, the molecular mechanisms by which PTER exerts its anticancer effects against acute myeloid leukemia (AML) cells were investigated. Methodology and Principal Findings: Results showed that PTER suppressed cell proliferation in various AML cell lines. PTER-induced G0/G1-phase arrest occurred when expressions of cyclin D3 and cyclin-dependent kinase (CDK)2/6 were inhibited. PTER-induced cell apoptosis occurred through activation of caspases-8-9/-3, and a mitochondrial membrane permeabilization (MMP)-dependent pathway. Moreover, treatment of HL-60 cells with PTER induced sustained activation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK)1/2, and inhibition of both MAPKs by their specific inhibitors significantly abolished the PTER-induced activati; | 2014 | 2014-10-06T23:46:41Z |
| Pathway of Programmed Cell Death and Oxidative Stress Induced by ?-Hydroxybutyrate in Dairy Cow Abomasum Smooth Muscle Cells and in Mouse Gastric Smooth Muscle. | This article is from PLoS ONE , volume 9 . Abstract The administration of exogenous β-hydroxybutyrate (β-HB), as well as fasting and caloric restriction, is a condition associated with β-HB abundance and decreased appetite in animals. Increased β-HB and decreased appetite exist simultaneously in some diseases, such as bovine left displaced abomasums (LDA) and human chronic gastritis. However, the effects of β-HB on stomach injuries have not been explored. To elucidate the possible effects of exogenous β-HB on the stomach, mice were injected intraperitoneally with β-HB, and bovine abomasum smooth muscle cells (BSMCs) were treated with different concentrations of β-HB. We found that β-HB induced BSMCs endoplasmic reticulum- and mitochondria-mediated apoptotic cell death. β-HB promoted Bax expression and caspase-12, -9, and -3 activation while blocking Bcl-2 expression. β-HB also promoted AIF, EndoG release and p53 expression. β-HB acted on key molecules in the apoptotic cell ; | 2014 | 2014-10-22T19:40:13Z |
| Dual Control of Yen1 Nuclease Activity and Cellular Localization by Cdk and Cdc14 Prevents Genome Instability. | This article is from Molecular Cell , volume 54 . Abstract The careful orchestration of cellular events such as DNA replication, repair, and segregation is essential for equal distribution of the duplicated genome into two daughter cells. To ensure that persistent recombination intermediates are resolved prior to cell division, the Yen1 Holliday junction resolvase is activated at anaphase. Here, we show that the master cell-cycle regulators, cyclin-dependent kinase (Cdk) and Cdc14 phosphatase, control the actions of Yen1. During S phase, Cdk-mediated phosphorylation of Yen1 promotes its nuclear exclusion and inhibits catalytic activity by reducing the efficiency of DNA binding. Later in the cell cycle, at anaphase, Cdc14 drives Yen1 dephosphorylation, leading to its nuclear relocalization and enzymatic activation. Using a constitutively activated form of Yen1, we show that uncontrolled Yen1 activity is detrimental to the cell: spatial and temporal restriction of Yen1 protects against g; | 2014 | 2014-10-23T07:40:09Z |
| Protein kinase C signaling and cell cycle regulation. | This article is from Frontiers in Immunology , volume 3 . Abstract A link between T cell proliferation and the protein kinase C (PKC) family of serine/threonine kinases has been recognized for about 30 years. However, despite the wealth of information on PKC-mediated control of, T cell activation, understanding of the effects of PKCs on the cell cycle machinery in this cell type remains limited. Studies in other systems have revealed important cell cycle-specific effects of PKC signaling that can either positively or negatively impact proliferation. The outcome of PKC activation is highly context-dependent, with the precise cell cycle target(s) and overall effects determined by the specific isozyme involved, the timing of PKC activation, the cell type, and the signaling environment. Although PKCs can regulate all stages of the cell cycle, they appear to predominantly affect G0/G1 and G2. PKCs can modulate multiple cell cycle regulatory molecules, including cyclins, cyclin-dependent kin; | 2013 | 2014-10-28T20:08:08Z |
| B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. | This article is from Cell Death & Disease , volume 4 . Abstract B-Myb is a highly conserved member of the Myb transcription factor family that has essential roles in cell-cycle progression. Recent work has suggested that B-Myb is also involved in the cellular DNA-damage response. Here, we have investigated the fate of B-Myb in UV-irradiated cells. UV stress leads to the appearance of phosphorylated B-Myb in nuclear SC35 speckles during transcriptional shutdown. Furthermore, we show that UV irradiation leads to a change of the phosphorylation pattern of B-Myb, which is caused by a switch from Cyclin/Cdk-dependent to Jnk and p38 kinase-dependent phosphorylation. Taken together, we have identified Jnk and p38 kinase as novel regulators of B-Myb and established the localization of phosphorylated B-Myb in SC35 speckles as a potential novel regulatory mechanism for B-Myb in UV irradiated cells. | 2013 | 2014-10-24T22:51:18Z |
| Teaching and Learning Adolescent Psychiatry | | 1971 | 2025-09-26T19:28:34Z |
| Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4. | This article is from PLoS Genetics , volume 10 . Abstract DNA double-strand breaks (DSBs) can be repaired by one of two major pathways—non-homologous end-joining (NHEJ) and homologous recombination (HR)—depending on whether cells are in G1 or S/G2 phase, respectively. However, the mechanisms of DSB repair during M phase remain largely unclear. In this study, we demonstrate that transient treatment of M-phase cells with the chemotherapeutic topoisomerase inhibitor etoposide induced DSBs that were often associated with anaphase bridge formation and genome instability such as dicentric chromosomes. Although most of the DSBs were carried over into the next G1 phase, some were repaired during M phase. Both NHEJ and HR, in particular NHEJ, promoted anaphase-bridge formation, suggesting that these repair pathways can induce genome instability during M phase. On the other hand, C-terminal-binding protein interacting protein (CtIP) suppressed anaphase bridge formation, implying that CtIP fu; | 2014 | 2014-10-06T07:32:29Z |
| PD-0332991 induces G1 arrest of colorectal carcinoma cells through inhibition of the cyclin-dependent kinase-6 and retinoblastoma protein axis. | This article is from Oncology Letters , volume 7 . Abstract Preclinical and clinical studies have demonstrated the anticancer activity of PD-0332991, a selective cyclin-dependent kinase 4/6 (CDK4/6) inhibitor, in the treatment of various types of cancer in a retinoblastoma protein (RB)-dependent manner. However, it remains unclear whether CDK4, CDK6 or both are required for RB phosphorylation in colorectal carcinoma and thus PD-0332991 can be used to target this CDK-RB axis for the cancer therapy. The aim of this study was to determine whether CDK4, CDK6 and phosphorylated RB proteins were overexpressed in colorectal carcinoma tissues as compared to matched normal colorectal tissues. The results showed that knockdown of CDK6 but not CDK4 reduced RB phosphorylation and inhibited carcinoma cell growth. Thus, CDK6 plays a critical role in RB phosphorylation and cancer growth. PD-0332991 treatment blocked RB phosphorylation and inhibited cell growth through the induction of G1 arrest of co; | 2014 | 2014-10-23T06:07:14Z |
| github.com-DovAmir-awesome-design-patterns_-_2021-07-10_17-58-07 | A curated list of software and architecture related design patterns. Awesome Software and Architectural Design Patterns A curated list of software and architecture related design patterns. Software design pattern - A general, reusable solution to a commonly occurring problem within a given context in software design. It is a description or template for how to solve a problem that can be used in many different situations. Contents Programming language design patterns General Architecture Cloud Architecture Serverless Architecture Micro services & Distributed Systems Internet of things Big Data Databases and storage Docker and DevOps Mobile Front End Development Security Books Programming Language Design Patterns AngularJS design patterns C# design-patterns design-patterns-for-human - ultra simplified explanation to design patterns. Design Patterns Library C++ design-patterns Closure design-patterns Go design-patterns Java sourcemaking - patterns and anti patterns. oodesign - patterns ca; | 2021 | 2021-09-22T22:10:51Z |
| Ep 10 Part 2: Kristine Bunch's Struggle For Freedom | In Part 2 of this episode, Kristine and her newly-formed defense team fight to win back her freedom. Armed with advances in fire science, a potential Brady violation, and some shocking revelations about the State's evidence at trial, Kristine and her team fight till the very end. A special thank you to Kristine Bunch for helping me tell her story. Please consider supporting Court Junkie with as little as $3 a month via Patreon.com/CourtJunkie. Help support Court Junkie with $6 a month and get access to bonus monthly episodes.Follow me on Twitter @CourtJunkiePod or Instagram at CourtJunkie. Music featured in this episode: Like Music (cdk Mix) by cdk (c) copyright 2015 Licensed under a Creative Commons Attribution Share-Alike (3.0) license. http://dig.ccmixter.org/files/cdk/48915 Ft: Phasenwandler Homesick (Keymix) by keytronic (c) copyright 2014 Licensed under a Creative Commons Attribution (3.0) license. http://dig.ccmixter.org/files/Keytronic/47457 Ft: Kizzylotus Paper Planes - Durd; | 2016 | 2023-07-08T12:43:12Z |
| CIA Reading Room cia-rdp91-00929r000100440035-5: FR:ZAVROV M.I. TO:LAVROVSKIY M.P. | ProNems 0 Thawy 4 P U A 4 0 - W4narum=adon 45yalem of Guidame of AW-AbTrAft ArdbM Fire),by N, F. LAIMRU.~,aMv bk, Kv.~ TouU P U A Z Qm MOSWW*1960. pq 1-Ul -OVTI)--Mi:~~75us"MilApr 65C j 2.=duced in Underarcund OPtniflentiv,x; byLavrovp ct al,Dept of iftUS Bur of VArms~Centrsl ftyer Staft. Ro 342D-6?7 73'by 59XXXZMNLM ACeliMatizaticM of MM3lSrUtl3 IU thtUSSR., by H - P - JAvro _A 150 pp e___yWMIAN, bkl AM] 4 tizatsUa Qmdatry v MR$Isdatellotvo Teentrosoyatao 1957-Dept of UteriorM C3 V 7Sai - Biol 14 k, z a ~?sop 61RusBinn Game Reports. Vol II. Muskrat, 1951-1955.Fluctuations and Forecasts of Muskrat Numbers, byN. F. Levrov.RUSSIAN, per, Voprosy Biologii Pushnykh ?verey iTekhniki Ckhotnichlyego Promysta (USSR), V07 xi-XIV, 1051-1955.Order from Queen's PrinterCanadaSci - BiclJul 59CTS 1, 12Russian Game OReports. Vol II. Muskrat, 1951-10,55.Interiml nnd External Parasites of Muskrats, byN. F. Lavrov.RUSSIPJ;, per, Voprosy Biologii Pushnykh Zverey iTekhniki Oldiotnichlyego Promysta (USSR), V; | 1983 | 2026-04-02T12:22:45Z |
| DTIC ADA407257: The Role of the Suppressor, Rsu-1, in Regulation of Ras Signal Transduction in Breast Cancer Cells | Rsu-1 is a cDNA previously identified as an inhibitor of Ras-induced transformation. An HA-epitope tagged Rsu-1 cDNA was introduced into the MCF7 breast carcinoma cell line. Stable transfectants were selected for analysis of effects of Rsu-1 expression on growth control and Ras-dependent kinase pathways. Assessment of biological activity of HA-Rsu-1 transfectants revealed slower anchorage dependent growth rates , a significant reduction in anchorage independent growth and increased apoptosis compared to control MCF7 cell lines. Analysis of cell cycle regulatory proteins required for transit through 61 revealed that HA-Rsu-1 transfectant cell lines expressed elevated levels of p21 (CIP) CDK inhibitor but not cyclin D or p27 KIP, This appears to be due to stabilization of P53 by Rsu-1 expression as revealed by pulse-chase experiments. Moreover, Rsu-1 transfectants exhibited reduced levels of cyclin D- associated kinase and cdk2 kinase activity. Perturbations in signal transduction pathwa; | 2001 | 2018-05-09T18:17:52Z |
| github.com-localstack-localstack_-_2022-11-12_23-39-58 | 💻 A fully functional local AWS cloud stack. Develop and test your cloud & Serverless apps offline! LocalStack provides an easy-to-use test/mocking framework for developing Cloud applications. Overview • Install • Example • Run • Usage • Changelog • Contributing – 📖 Docs • 💻 Pro version • ☑️ Feature coverage • 📢 Announcements Overview LocalStack 💻 is a cloud service emulator that runs in a single container on your laptop or in your CI environment. With LocalStack, you can run your AWS applications or Lambdas entirely on your local machine without connecting to a remote cloud provider! Whether you are testing complex CDK applications or Terraform configurations, or just beginning to learn about AWS services, LocalStack helps speed up and simplify your testing and development workflow. LocalStack supports a growing number of AWS services, like AWS Lambda, S3, Dynamodb, Kinesis, SQS, SNS, and many more! The Pro version of LocalStack supports additiona; | 2022 | 2022-11-13T09:19:09Z |
| Recall Ft Kara Square (Stems) | Recall - Vocals by Kara Square (c) 2013 Licensed under a Creative Commons Attribution Noncommercial (3.0) license. http://dig.ccmixter.org/files/mindmapthat/43023 Ft: CDK The vocals to this Secret Mixter remix of CDK. Thank you to CDK for the inspiration. Here is a link to CDK's sample pack. WORDS I could bare my soul Tell you things I’ve held on for so long I could let it go Be the way I wish I were But you won’t recall Anything, anything, anything You won’t remember Anything, anything, anything When will you forget Everything? There are things that hurt I used to think you chose to forget them Things you don’t remember I learned to block things out by the best man But you won’t recall Anything, anything, anything You won’t remember Anything, anything, anything When will you forget Everything? Guilt and denial Have been your prison for so, so long Maybe you’ll break free As you continue to lose your memory But you won’t recall Anything, anything, anything You w; | | 2016-05-06T23:35:17Z |
| CIA Reading Room cia-rdp82-00457r002300710003-9: ATTEMPT OF TRUJILLO TO OBTAIN U.S. CURRENCY | 25X1A2g CENTRAL INTE ENCE AGENCY REPORT N INFORMATI N REPORT CD NO. Sanitized - Approved Fo si (~IA-RDP82-00457R002300 CLASa1FICATIO 4 SE -T COUNTRY Dominican Republic/Haiti, same plane with Fred Imbert.) SUBJECT Attempt of Trujillo to obtain U. S. Currency - NO: OF PAGES 1 PLACE ACQUIRED DATE OF IN 25X1X6 25X1 A6 < WARNING NGTI : TH S DIS"IZsZ i'= LISTING MUST BE EXCIS#D ~3I:r .E PUBLIC RLLI"A51" OF THIS DOCUMENT. DATE DISTR. 9 February 1949 NO. OF ENCLS. (LISTED 9#LOW) 25X1A6a SUPPLEMENT TO REPORT NO. to It is reported that on the morning of 31 January 1949, Anselmo Paulin, Dominican Secretary of State, without portfolio, personally called at the home of Manuel Imbert, prominent Dominican banker, and requested him to obtain 120,000 in United States currency as soon as possible. Paulin advised that Vo money was urgently needed by President Trujillo. 2, During the afternoon of 31 January, Fred Imbert, brother of Manuel and a resident of San Juan, Puerto Rico, arrived in Ciudad Trujillo; | 1949 | 2023-06-28T00:44:17Z |
| Crazy Bytes 4 - Forever | NOTE 2: @gyromatical repacked the RARs into unpassworded ZIPs for easy access here . Note: Since this has been giving so many people trouble, here are explicit instructions on how to use the discs. Run: cbytes4.com #4 Password: SUPERMAN It will ask you to insert all discs, then the one you want to access. You either need to download all 4, or to bypass the check try downloading CB4.CHK and placing it in your root directory, i.e if you are using DOSBox, whatever folder you have mounted as C. Originally posted to alt.binaries.warez by Haags Neesje. Front cover from pentagon.interbox.net. CD 1 - Games 1830 : RAILROADS & ROBBER BARONS (C) SIMTEX 1830 : RAILROADS & ROBBER BARONS MONEY PATCH 3D CYBERPUCK : [+4] TRAINER 3D CYBERPUCK : [+9] TRAINER 3D CYBERPUCK V1.1 (C) HOMEBREW SOFTWARE BEAT THE HOUSE (C) SPIRIT OF DISCOVERY BLOOD BOWL (C) MICROLEAGUE BLOOD BOWL : MODEM PATCH BOLO (C) DONGLEWARE CD - MULTILANGUAGE BOLZ! (C) COGNITION BRETT HULL HOCKEY (C) ACCOLADE BRIDGE DELUXE II (C) INTERPL; | 1995 | 2017-05-12T09:34:10Z |
| CIA Reading Room cia-rdp91-00772r000200890017-1: A FR: JOURNAL OF PHYSIQUE ET LE RADI TO: JOURNAL FUR PRAKTISCHE CHEMIE | Friu=.;-, by J. 7-van. U11CLASSIM-Dyer,'~ Journal of Phyal-quo et Le Radium. No-----------195'i7-Havy Tr 1.631/091-,127A E- C~Sci V410=10S.-iNb rDie Y90. TM trunaltion 0+---*~ 0:w r, lmdt of flwzt by U. T. Yuwmj14. Lab Igue-Frolow.. 15 pp.J. php. at :nLdlum4Hge 18, 48-P It98 15 dVth;b;r, 3,9'57.AND-tr-33MMA 58-505Sal 5P4 4~eAug 59pI irpriROF YICIHoilin~th hdo ~)y R-, Duverney,de'Physique et le Radium,16.!- 92261509270s611R-383-IqPreliininary;Ebcperiments on Radioactivities Consequentto J~xp.~osioa of Wires Containing D uterim, ByM. Bon~asFRENCH, per, Le Journal de Physique et le Radiixr,, Vol.2XVIII, No 1.0, 1957, PP 5E5-592*JP.RS for Army Rocket wid Guded Missile AAgency$7 SeP~ GOboalwaima Selector far slow Ifeutronsp by 0. GobertpD4 Tap---otp 21 pp.jrvaival dm- !I~T 10 Ruium.Val M 19501, -PP51-A-,wjuts 6.36aLqiulatlona of the Us *am Ymber of NeutronsEmitted by IndWed Flosion of u23tJ by Ifeutrowiof 11F.2 I%Vp by S. Alalse, M. Gaudin, at &I.FRMII., Oarj, Joamal 62 Rtyslque et le Radiu; | 1983 | 2026-03-25T23:48:15Z |
| Remarkable women of france ( from 1431 to 1749 ) | | 1914 | 2025-09-08T04:07:29Z |
| ＰＨＯＮＫ ＦＲＩＤＡＹＳ Season 2 Ep. 1 : SUMMER OF 88 | -PHONK FRIDAYS SEASON 2 Episode 1 …SUMMER OF 88: THE ENDLESS SUMMER… mixed by iNFMS -special thanks: www.badradio.nz, the discord bois. The entire LSF crew. Analog by Nature, the_accidental_poet and Ryan Celsius.- ------------SUMMER OF 88------------- - 00:12 system - DONT MAKE A MOVE https://soundcloud.com/sys32sys32 03:48 otto22 - FOR THE LAST TIME https://soundcloud.com/otto22beats 06:00 EXISTANZE - AT ALL COSTS https://soundcloud.com/existanze 09:00 swxy - hollow tips https://soundcloud.com/swxybeatz 11:48 Re_V - MURDA SHOW w/ sword https://soundcloud.com/revbeatz https://soundcloud.com/sworddy 13:45 E R R O R - RIP NIPSEY w/ ABO https://soundcloud.com/3rr0r404 https://soundcloud.com/mahatmaganja420 17:51 STVSHBOI - EZ COME EZ GO w/ iNFMS https://soundcloud.com/stvshboi https://soundcloud.com/iaminfms 20:08 sxlvr - CODEINE SIPPIN w/ iNFMS https://soundcloud.com/sxlvr https://soundcloud.com/iaminfms 23:25 GREEN PICCOLO - Hollywood https://soundcloud.com/greenpiccolobeats 26:53 c; | 2019 | 2024-02-13T18:26:16Z |
| Barts Installer #14 - Super Language Collection | Looking for old abandonware warez CD ISO's - If you have any not in my collection please contact me (pascal_of_irate @ hotmail . com). I can remove any copy protections from them (if required) and repost cracked versions for the community to enjoy. I am especially interested in CD's from the likes of Blobby International, Playdoh, Voodoo, Ghost, Tango, Delta, USA, Gold, Redhot, Jurassic, Pepsi, Alkey, Capaccino, Coke, Total, Razor, Xenon etc. but also any others from this era. Please see our special requests as the bottom of this page. BART`s INSTALLER 14 SUPER LANGUAGE COLLECTION BC50 [DIR] BORLAND C++ V5.0 DEVELOPERS SUITE COBOL [DIR] MS COBOL V4.5 CPU_CTL [DIR] SPECIAL COLLECTION FOR ELECTRONIC HOBBISTS DELPHI20 95 [DIR] BORLAND DELPHI 2.0 DEVELOPERS SUITE FORTH [DIR] FORTH V2.0 FORTRAN [DIR] MS FORTRAN V5.1 FOXLIB26 [DIR] MS FOXPRO 2.6 LIBRARY CDK FOXPODBC [DIR] MS FOXPRO 2.6 ODBC FOXPRO26 DOS [DIR] MS FOXPRO 2.6 FOR DOS MASM6 [DIR] MS MACRO ASSEMBLER 6.0 MSC70 [DIR] MS C/C++ V7.0 ; | | 2019-11-09T21:22:32Z |
| github.com-localstack-localstack_-_2023-09-29_02-11-07 | 💻 A fully functional local AWS cloud stack. Develop and test your cloud & Serverless apps offline :zap: Join the LocalStack Insiders program to help us shape the future of LocalStack! Test drive new features & engage in conversations with us so we can learn from your use cases :zap: LocalStack provides an easy-to-use test/mocking framework for developing cloud applications. Overview • Install • Example • Run • Usage • Releases • Contributing 📖 Docs • 💻 Pro version • ☑️ LocalStack coverage Overview LocalStack is a cloud service emulator that runs in a single container on your laptop or in your CI environment. With LocalStack, you can run your AWS applications or Lambdas entirely on your local machine without connecting to a remote cloud provider! Whether you are testing complex CDK applications or Terraform configurations, or just beginning to learn about AWS services, LocalStack helps speed up and simplify your testing and development workflow. LocalStack s; | 2023 | 2023-09-29T05:13:28Z |
| github.com-T-head-Semi-wujian100_open_-_2019-10-24_06-20-55 | IC design and development should be faster，simpler and more reliable wujian100_open wujian100_open is a MCU base SoC. We can simulate by EDA tools and emulate by FPGA. Also we can develop the IPs and software in this platform. We wish more and more developers building the open MCU ecosystem with T-Head. IC design and development should be faster simpler and more reliableDirectory Structure|--Project //open source project work directory |--riscv_toolchain //tool chain install directory download from t-head.cn |--wujian100_open //wujian100_open project get from github |--case //test case example for simulation |--doc //wujian100_open user guide |--fpga //FPGA script |--lib //compile script for simulation |--regress //regression result |--sdk //software design kit |--soc //Soc RTL source code |--tb //test bench |--tools //simulation script and setup file |--workdir //simulation directory |--LICENSE |--README.md Get Start 1. prepare a project work directory just like 'Project'2. cd Proje; | 2019 | 2019-10-24T09:00:18Z |
| Cyclin-dependent kinases 7 and 9 specifically regulate neutrophil transcription and their inhibition drives apoptosis to promote resolution of inflammation. | This article is from Cell Death and Differentiation , volume 19 . Abstract Terminally differentiated neutrophils are short-lived but the key effector cells of the innate immune response, and have a prominent role in the pathogenesis and propagation of many inflammatory diseases. Delayed apoptosis, which is responsible for their extended longevity, is critically dependent on a balance of intracellular survival versus pro-apoptotic proteins. Here, we elucidate the mechanism by which the cyclin-dependent kinase (CDK) inhibitor drugs such as R-roscovitine and DRB (5,6-dichloro-1-beta-𝒟-ribofuranosylbenzimidazole) mediate neutrophil apoptosis. We demonstrate (by a combination of microarray, confocal microscopy, apoptosis assays and western blotting) that the phosphorylation of RNA polymerase II by CDKs 7 and 9 is inhibited by R-roscovitine and that specific effects on neutrophil transcriptional capacity are responsible for neutrophil apoptosis. Finally, we show that specific CDK7 and 9 i; | 2012 | 2014-10-26T21:30:14Z |